Monoclonal antibodies to p65, and phospho-specific p65-NF-κB, MMP-9, CXCR4, cleaved-Caspase-3, and β1-Integrin, were obtained from R&D Systems (Heidelberg, Germany). Antibodies to β-actin were from Sigma-Aldrich (Taufkirchen, Germany). Secondary rhodamine-coupled antibodies for immunofluorescence and anti-Ki67 were from Dianova (Hamburg, Germany), and alkaline phosphatase-linked antibodies for Western blotting were from EMD Millipore (Schwalbach, Germany). Monoclonal anti-ALDH1 was obtained from Acris Antibodies GmbH (Herold, Germany). Monoclonal anti-CD133 and anti-CD44 were purchased from Abcam PLC (Cambridge, UK). MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), 4′,6-diamidino-2-phenylindole (DAPI), and alginate were from Sigma-Aldrich (Taufkirchen, Germany). TNF-β was purchased from eBiosciences (Frankfurt, Germany). Furthermore, TNF-β was given as a kind gift by Genetech (South San Francisco, CA, USA). Calebin A was a generous gift from Sabinsa Corporation (East Windsor, NJ, USA). Calebin A was diluted as 10,000 µM stock solution in dimethyl sulfoxide (DMSO) and further diluted in cell culture medium for experimental investigations. The final concentration of DMSO did not exceed 0.1% during the experiments.
Antibodies to β actin
Manufactured by Merck Group
Sourced in Germany
Antibodies to β-actin are a type of protein that specifically bind to the β-actin protein, which is a common structural component found in the cytoskeleton of all eukaryotic cells. These antibodies can be used in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect and quantify the presence of β-actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cxcr4, by R&D Systems (2 mentions)
Integrin β1, by R&D Systems (2 mentions)
Secondary rhodamine coupled antibodies for immunofluorescence and anti ki67, by Dianova (2 mentions)
Alkaline phosphatase linked antibodies for western blotting, by Merck Group (2 mentions)
Tnf β, by Thermo Fisher Scientific (2 mentions)
Tnf β, by Gene Tech (2 mentions)
Cleaved caspase 3, by R&D Systems (2 mentions)
Mmp 9, by R&D Systems (2 mentions)
Anti mouse alexa594, by Thermo Fisher Scientific (2 mentions)
Cyclin d1, by Cell Signaling Technology (2 mentions)
Fluorescein lotus tetragonolobus lectin, by Vector Laboratories (2 mentions)
Anti rabbit hrp and anti mouse hrp secondary antibodies, by Vector Laboratories (2 mentions)
Anti rabbit dylight 488, by Vector Laboratories (2 mentions)
Phospho 4ebp1, by Cell Signaling Technology (2 mentions)
Cyclin e1, by Cell Signaling Technology (2 mentions)
Rapamycin, by LC Laboratories (2 mentions)
Alginate, by Merck Group (1 mentions)
Bms 345541, by Merck Group (1 mentions)
Phospho specific p65 nf κb, by R&D Systems (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
16 protocols using antibodies to β actin
1
Monoclonal Antibodies and Cellular Targets in Cancer Research
2
Molecular Markers Analysis in Cancer
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Monoclonal antibodies to p65, as well as phospho-specific p65-NF-κB, MMP-9, CXCR4, cleaved-Caspase-3, β1-Integrin and PARP, were obtained from R&D Systems (Heidelberg, Germany). Antibodies to β-Actin were from Sigma-Aldrich (Taufkirchen, Germany). Secondary rhodamine-coupled antibodies for immunofluorescence and anti-Ki67 were from Dianova (Hamburg, Germany) and alkaline phosphatase-linked antibodies for Western blotting from EMD Millipore (Schwalbach, Germany). MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DAPI, 5-Fluorouracil (5-FU), alginate, BMS-345541 and dithiothreitol (DTT) were from Sigma-Aldrich (Taufkirchen, Germany). Stock solutions of BMS-345541 (1000 µM) and of DTT (1000 mM) were prepared in PBS and further diluted in normal cell culture growth medium to obtain final concentrations. Then, 5-FU was diluted as a 1000 µM stock solution in ethanol and further diluted in the cell culture medium. Final concentrations of ethanol did not exceed 0.1% during treatment. TNF-β was purchased from eBiosciences (Frankfurt, Germany). Further, TNF-β was given as a kind gift by Genetech (South San Francisco, CA, USA). Calebin A (CA), with a purity of 99.65%, was a generous gift from Sabinsa Corporation (East Windsor, NJ, USA). Epon for electron microscopy was purchased from Plano (Marburg, Germany).
3
Molecular Markers in Kidney Disease
4
Molecular Markers in Kidney Disease
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Antibodies against total rpS6, phospho-rpS6, Cyclin D1, Cyclin E1, and phospho-4E-BP1 were from Cell Signaling Technology (Beverly, MA). Fluorescein Lotus-tetragonolobus Lectin (LTL) was purchased from Vector Laboratories Inc, (Burlingame, CA). Anti-synaptopodin antibody was from Acris Antibodies GmbH (San Diego, CA). Anti-rabbit HRP and anti-mouse HRP secondary antibodies, anti-rabbit dyLight 488 were from Vector laboratories (Burlingame, CA). Anti-mouse Alexa 594 was from Invitrogen Life Technologies (Carlsbad, CA). Rapamycin was purchased from LC Laboratories (Woburn, MA). Antibodies to β-actin and other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO).
5
Sirtuin-Mediated Regulation of Cancer Cells
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All cancer and normal cells lines were obtained from the American Type Culture Collection (Manassas, VA). LNCaP, PC3, Ramos, Jurkat, H1299 and MRC5 cells were maintained in RPMI-1640 medium with 10% FBS (HyClone, CO). H460, A549, ZR75 and MDA231 cells were maintained in DMEM medium with 10% FBS. PZ-HPV-7 cells were maintained in Keratinocyte Serum-Free Medium supplemented with Epidermal Growth Factor (Invitrogen, Carlsbad, CA).
Antibodies to SIRT1 (sc-74504) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies to Ac-p53, p53, Ac-Histone H4 and H4 were purchased from Millipore (Billerica, MA). Antibodies to β-actin were purchased from Sigma-Aldrich (St. Louis, MO). Sirtinol was purchased from Sigma-Aldrich.
Antibodies to SIRT1 (sc-74504) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies to Ac-p53, p53, Ac-Histone H4 and H4 were purchased from Millipore (Billerica, MA). Antibodies to β-actin were purchased from Sigma-Aldrich (St. Louis, MO). Sirtinol was purchased from Sigma-Aldrich.
6
Western Blot Protein Analysis Protocol
7
Androgen Receptor Signaling in Cell Adhesion
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Chemicals, unless indicated otherwise, were from Sigma Aldrich (St. Louis, MO, USA). Synthetic androgen R1881 was from Sigma Aldrich, extracellular matrices fibronectin, vitronectin, laminin, collagen I, collagen IV and Src inhibitor PP2 were from Millipore (Billerica, MA, USA). Cycloheximide (Sigma-Aldrich) and MG132 (EMD-Millipore, Billerica, MA, USA) were used at indicated concentrations. Antibodies to AR (N-20), Integrin β1 (M-106) and Filamin A were from Santa Cruz Biotechnology (Santa Cruz, CA, USA), Rac-1 (clone 102) was from BD Biosciences (San Jose, CA, USA). Total FAK, phospho-FAK (Y397), total Src, phospho-Src (Y416) were from Cell Signaling, Inc. (Danvers, MA, USA). RNase L monoclonal antibody was kindly provided by Robert Silverman (Cleveland Clinic). Antibodies to β-actin, monoclonal and polyclonal antibodies to Flag tag, Flag-M2 agarose beads were from Sigma Aldrich. Anti-mouse IgG and anti-rabbit IgG HRP linked secondary antibodies were from Cell Signaling, Inc. (Danvers, MA, USA) and ECL reagents were from GE Healthcare (Piscataway, NJ, USA) and Boston Bioproducts (Ashland, MA, USA). Alexa 488-labeled Phalloidin, and Alexa fluor 647 donkey anti-rabbit IgG were from Life Technologies, Carlsbad, CA, USA.
8
Antibody-based analysis of mitochondrial proteins
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Antibodies to Parkin, PINK1, MFN1, and MFN2 were acquired from Cell Signaling Technology. The antibody to Parkin used for IHC was from LSBio and used at 1:500 dilution. Antibodies to TKT and vinculin were from Abcam. Antibodies to Src, Tyr416-phosphorylated Src, FAK, and Tyr925- or Tyr397-phosphorylated FAK were from Cell Signaling Technology. Antibodies to β-actin and RHOT1 were from Sigma-Aldrich and Santa Cruz Biotechnology, respectively. MitoTracker Green, Phalloidin Alexa Fluor 488, MitoTracker Deep Red FM, and secondary antibodies used in immunofluorescence studies were from Molecular Probes. FCCP, MitoTempo, and etoposide were purchased from Sigma-Aldrich. BafA was purchased from Cell Signaling Technology. NADPH was determined by PicoProbe NADPH quantification fluorometric assay kit from BioVision. Kits for glucose uptake, lactate production, and TKT activity were purchased from BioVision.
9
Characterization of Antibodies for Proteomics
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Hexavalent Chromium (potassium dichromate solution) and antibodies to β‐actin were purchased from Sigma–Aldrich® (Basel, Switzerland). Antibodies to nanog, Poly (ADP‐ribose) polymerase (PARP), and vimentin were acquired from Cell Signaling Technology, Inc. (Danvers, MA). Antibodies to alpha smooth muscle actin (α‐SMA) and paxillin were obtained from Thermo Fisher Scientific, Inc. (Waltham, MA). Antibodies to CD‐133, Src, and connexin‐43 (CX‐43) were respectively bought from Biorbyt (Cambridge, Cambridgeshire, UK), Calbiochem‐Merck Biosciences, Merck Millipore (Taipei, Taiwan), or Santa Cruz Biotechnology, Inc. (Dallas, TX). Monoclonal antibodies to AIF, ATAD3A, DDH, DRP1, eEF2 12 , 13 , 14 , 15 , 16 , Mfn‐2, and SAE2 (Supporting information Figure S1) were home‐raised against the respective recombinant protein. The monoclonal antibodies were characterized by immunoblotting, which recognized the proteins with particular molecular weight in a gel electrophoresis of the A549 cell lysates. Proteins isolated by immunoprecipitation were further analyzed by MALDI‐TOF, and the identity of the protein was determined by peptide mass fingerprint‐matching to the specific protein sequence in the database of GenBank (www.ncbi.nlm.nih.gov/genbank ) and UniProtKB/Swiss‐Prot (www.ebi.ac.uk/swissprot/ ).
10
Evaluating Antioxidant Pathways
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