H600l microscope

Manufactured by Nikon

Sourced in Japan, United States

The Nikon H600L microscope is a high-quality optical instrument designed for laboratory and research applications. It features a sturdy construction, advanced optics, and versatile functionality to meet the needs of scientific professionals. The H600L provides clear and detailed magnification of specimens, enabling precise observation and analysis.

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Spelling variants of this product

H600L light microscope (7 citations) H600L Microscope and image analysis system (3 citations) Nikon series Eclipse H600L microscope (2 citations) Nikon C2 H600L confocal microscope (1 citations)

32 protocols using h600l microscope

Evaluating Mitochondrial Damage by AgNPs

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To analyze the mitochondrial damage induced by exposure to AgNPs, mitochondrial membrane potential (MMP) analysis and mitochondrial superoxide analysis were performed by using THP-1 cells. For MMP analysis, THP-1 cells were exposed to 1 µM PLGA-PTE for 1 h prior to exposure to 6 µg/mL AgNPs. After 6 h of exposure, the level of MMP was measured using the staining of MitoTracker^® Red CMXRos probe (M5712, Invitrogen, Rockford, IL, USA). Cells were treated with 100 nM MitoTracker^® Red CMXRos and incubated at 37 °C for 30 min and the nuclei were visualized by Hoechst 33258. Images were obtained with Nikon H600L microscope (Nikon, Japan) and the relative fluorescent intensity was measured by ImageJ software.
For mitochondrial superoxide analysis, THP-1 cells were exposed to 1 µM PLGA-PTE for 1 h prior to exposure to 6 µg/mL AgNPs. After 1 h of exposure, the level of mitochondrial superoxide was measured using MitoSOX™ Red probe (MM36008, Invitrogen, Rockford, IL, USA). The images were obtained with Nikon H600L microscope (Nikon, Japan) and the relative fluorescent intensity was measured by ImageJ software (https://imagej.nih.gov/ij/download.html).

Chen R.J., Huang C.C., Pranata R., Lee Y.H., Chen Y.Y., Wu Y.H, & Wang Y.J. (2021). Modulation of Innate Immune Toxicity by Silver Nanoparticle Exposure and the Preventive Effects of Pterostilbene. International Journal of Molecular Sciences, 22(5), 2536.

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Microscopic Examination of Cancer Cell Morphology

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Morphological changes in MCF-7 and MDA-MB231 cells, treated with 50 and 100 µg/ml concentrations of BTB extract for 48 h, were observed under inverted contrast microscope (Nikon H600L microscope; Nikon, Japan) with suitable filter 20× and 40× magnifications consecutively. The morphological changes in the cells were also examined by staining with Giemsa (Merck, USA) for 10 min and observed under the phase contrast microscope (Nikon H600L microscope), as detailed by Chih et al. [15 (link)].

Kumar S., Sharma V.K., Yadav S, & Dey S. (2017). Antiproliferative and apoptotic effects of black turtle bean extracts on human breast cancer cell line through extrinsic and intrinsic pathway. Chemistry Central Journal, 11, 56.

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Immunofluorescence Staining of Neurons

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VGNs were plated on glass slides and fixed with 4% paraformaldehyde in PBS for 2 hours at 4°C, permeabilized with 0.2% Triton X-100. Mouse antineurofilament 200 (NF200) (1 : 500, Sigma) and rabbit anti-COX-1/COX-2 (1 : 300, Abcam) were added to the cells at 4°C overnight. Then cells were incubated in Rhod (TRITC) AffiniPure donkey anti-rabbit or Alexa Fluor 488 donkey anti-mouse or Rhod AffiniPure donkey anti-mouse secondary antibody (1 : 500, Invitrogen) for 1 hour at 37°C and were counterstained with DAPI (1 : 800, Invitrogen) for 15 minutes to stain nuclei prior to final embedding. Fluorescent images were obtained using a NIKON H600L microscope (Japan).

Liu Y., Li S, & Wang Z. (2014). The Role of Cyclooxygenase in Multiplication and Reactivation of HSV-1 in Vestibular Ganglion Neurons. The Scientific World Journal, 2014, 912640.

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Evaluating Mitochondrial Dynamics in gsr-1 Deficiency

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To determine if gsr-1 deficiency had any effect on mitochondria related phentoypes, age-synchronized, young adult animals (n = 60) were assessed for mitochondrial networking in gsr-1(m+,z-); ccIs4251 [Pmyo-3::mito::gfp] versus control ccIs4251 [Pmyo-3::mito::gfp] animals. There was significantly greater mitochondrial fragmentation in the gsr-1 animals than controls at 20°C (P <0.001) and 25°C (P <0.05). Determination of sarcomere structure in gsr-1(m+,z-); jIs01[Pmyo-3::myo-3::gfp] were compared to control jIs01[Pmyo-3::myo-3::gfp] animals (n = 20 per group) both at 20°C and at 25°C. There was no significant difference between groups (P >0.05). For mitochondrial content determination, we loaded animals with JC-10, a dye that accumulates proportionally to a negative mitochondrial membrane potential as previously described [79 (link)]. Wild type control animals had significantly greater fluorescence of the gut mitochondria than did gsr-1(m+,z-) animals (P <0.001). Images were taken with a fixed exposure of 1 s on the Nikon H600L microscope and the fluorescence was quantified using ImageJ. Corrected total animal fluorescence was calculated as follows: Integrated density – area of the worm x mean fluorescence of three background readings. Data were analyzed using a Mann-Whitney test as data were non-parametric.

Mora-Lorca J.A., Sáenz-Narciso B., Gaffney C.J., Naranjo-Galindo F.J., Pedrajas J.R., Guerrero-Gómez D., Dobrzynska A., Askjaer P., Szewczyk N.J., Cabello J, & Miranda-Vizuete A. (2016). Glutathione reductase gsr-1 is an essential gene required for Caenorhabditis elegans early embryonic development. Free radical biology & medicine, 96, 446-461.

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Stapled Peptide-Induced Foci Formation

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HT1080 cells were grown on poly-L-lysine coated coverslips in 24-well plates in complete media until they reached at least 60% confluence. Plates were washed twice with PBS and then stapled peptide at 6 μM concentration in OptiMEM media was added to the wells for 18 hours. Next, cells on the coverslip were first fixed with methanol for 20 minutes at −20 °C, followed by their rehydration, by washing twice them with PBS. Then, cells were incubated in 70% ethanol for 10 minutes at 4 °C and washed twice with PBS. Finally, Prolong Gold antifade reagent with DAPI (4′, 6-diamino-2-phenylindole, Molecular Probes) was mounted on cells and the coverslips were sealed with nail polish. Foci were examined in a Nikon H600L microscope. Randomly selected fields from 3 independent coverslips were examined.

Chatterjee N., D’Souza S., Shabab M., Harris C.A., Hilinski G.J., Verdine G.L, & Walker G.C. (2020). A Stapled POL κ Peptide Targets REV1 to Inhibit Mutagenic Translesion Synthesis.. Environmental and molecular mutagenesis, 61(8), 830-836.

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Immunohistochemical Analysis of Collagen Type II

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In addition to histomorphological evaluation, immunohistological analysis for collagen type II was performed in all sections. After deparaffinization and rehydration of the tissue sections, collagen type II was immunostained with the two-step immunohistochemistry method instructed by the manufacturer (Zhongshan Goldenbridge Biotechnology Co., Ltd, Beijing, China).
The sections were incubated with monoclonal mouse antirat collagen type II antibody (1:200 dilution, Fisher Scientific, Chicago, IL, USA) for 3.5 h at 29°C. The slides were washed in PBS three times, and followed by a 20-minute incubation at 37°C with anti-mouse IgG/HRP (Fisher Scientific) and visualized with DAB chromagen. The slides were stained for 40 s before the nuclei with hematoxylin were counterstained for 6 s. The collagen type II content was evaluated based on optical density measured using image analysis software (Nikon H600L Microscope and Image-Pro Plus 6.0 image analysis system).

Song J.Q., Dong F., Li X., Xu C.P., Cui Z., Jiang N., Jia J.J, & Yu B. (2014). Effect of Treadmill Exercise Timing on Repair of Full-Thickness Defects of Articular Cartilage by Bone-Derived Mesenchymal Stem Cells: An Experimental Investigation in Rats. PLoS ONE, 9(3), e90858.

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Germline GFP Desilencing Assay in C. elegans

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The exposure and GFP germline desilencing assessments were performed as previously described (Lundby et al., 2016 (link)). Briefly, all chemicals tested were obtained from Sigma-Aldrich and were dissolved in DMSO to a stock concentration of 100 mM. Worms were synchronized by bleaching an adult population of the strain of interest, plating the eggs, and allowing the synchronized population to reach L4 larval stage (approximately 50 hr). These were then collected and incubated for 48 hr in 50 μL OP50 bacteria, 500 μL M9, and 0.5 μL of the chemical of interest for a final chemical concentration of 100 μM. After 48 hr, the worms were collected and allowed to recover on NGM plates for 1–2 hr (mixed population) or immediately plated as individual worms to separately labeled 35-mm seeded NGM plates (GFP+/− population sorting) and recovered there. Worms were scored for germline GFP expression using a Nikon H600L microscope at 40× magnification.

Camacho J., Truong L., Kurt Z., Chen Y.W., Morselli M., Gutierrez G., Pellegrini M., Yang X, & Allard P. (2018). The Memory of Environmental Chemical Exposure in C. elegans Is Dependent on the Jumonji Demethylases jmjd-2 and jmjd-3/utx-1. Cell reports, 23(8), 2392-2404.

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Mandible Histomorphometric Analysis

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Harvested mandibles (first molar region) were fixed in 10% buffered formalin, then were decalcified in 10% EDTA for 2 months. After dehydration, mandibles were embedded in paraffin and cut into sagittal section of 5 µm for HE staining, HE examination to see the number of osteoclasts and osteoblasts seen in five visual fields. Image acquisition was performed using a Nikon H600L microscope under ×200, ×400, and ×1,000 magnification, DS-Fi2 camera, and Nikon image system software.

Hendrijantini N., Suisan Y.C., Megantara R.W., Tumali B.A., Kuntjoro M., Ari M.D., Sitalaksmi R.M, & Hong G. (2022). Bone Remodeling in Mandible of Wistar Rats with Diabetes Mellitus and Osteoporosis. European Journal of Dentistry, 17(2), 319-329.

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Optic Nerve Ultrastructural Analysis

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One month after IOP elevation, we perfused WT and Bax^-/- mice with 0.1 M cacodylate buffer followed by 2.5% glutaraldehyde in cacodylate buffer. We carefully removed eyes with the optic nerve attached. We then isolated 3-mm segments of optic nerve proximal to the globe and post-fixed them for 1 h in 2.5% glutaraldehyde in cacodylate buffer. Next, we embedded the optic nerve segments in Epon resin and obtained semi-thin (700 nm, light microscopy) and ultra-thin (70 nm, electron microscopy) cross-sections. We stained semi-thin cross-sections with 1% paraphenylenediamine (PPD; in a 1:1 mixture of methanol and 2-propanol) and 1% toluidine blue to identify myelin sheaths and glia, respectively. We imaged sections en montage using a Nikon H600L microscope equipped with a 100× oil-immersion objective, motorized X-Y-Z stage, a digital SLR camera, and differential interference contrast optics. Ultra-thin nerve cross-sections were prepared and photographed at 2700× (10 images per nerve for axon counts) and 240× magnification (one image per nerve to measure cross-sectional nerve area) using a Philips CM-12, 120-keV transmission electron microscope at Vanderbilt Cell Imaging Shared Resource Core. A naïve observer manually counted total and degenerating axons using Fiji ImageJ. We identified degenerating axons based on multilaminar myelin sheaths and diminishment of cytoskeletal content.

Risner M.L., Pasini S., McGrady N.R, & Calkins D.J. (2022). Bax Contributes to Retinal Ganglion Cell Dendritic Degeneration During Glaucoma. Molecular Neurobiology, 59(3), 1366-1380.

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Germline GFP Desilencing Assay in C. elegans

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