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Folin and ciocalteu s phenol reagent

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Folin and Ciocalteu's phenol reagent is a chemical solution used for the colorimetric determination of phenolic compounds. It contains a mixture of phosphomolybdic and phosphotungstic acids.

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Lab products found in correlation

Ethyl alcohol, by Thermo Fisher Scientific (2 mentions) Quercetin hydrate, by Thermo Fisher Scientific (2 mentions) Anhydrous sodium carbonate, by Thermo Fisher Scientific (2 mentions) Gallic acid monohydrate, by MP Biomedicals (2 mentions) Sodium acetate, by Merck Group (2 mentions) Potassium acetate, by Thermo Fisher Scientific (1 mentions) Ethanol, by Thermo Fisher Scientific (1 mentions) Xad7hp, by Merck Group (1 mentions) Milli q advantage a10 water purification system, by Merck Group (1 mentions) Optima lc ms grade, by Thermo Fisher Scientific (1 mentions) Analar normapur acs, by Avantor (1 mentions) Gallic acid monohydrate acs reagent, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions)

5 protocols using folin and ciocalteu s phenol reagent

1

Chemicals Used for Analysis

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Chemicals used for the analysis were obtained from Nacalai Tesque Inc. (Kyoto, Japan) and Wako Pure Industries Ltd. (Osaka, Japan). Folin and Ciocalteu's phenol reagent were purchased from MP Biomedicals (CA, USA), and ethylenediaminetetraacetic acid (EDTA) was obtained from DOJINDO (Kumamoto, Japan).
Wang Z., Okutsu K., Futagami T., Yoshizaki Y., Tamaki H., Maruyama T., Toume K., Komatsu K., Hashimoto F, & Takamine K. (2020). Microbial Community Structure and Chemical Constituents in Shinkiku, a Fermented Crude Drug Used in Kampo Medicine. Frontiers in Nutrition, 7, 115.
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2

Quantifying Polyphenol Content in Sweet Potatoes

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As standard solutions, gallic acid was adjusted to 500, 250, 100, 50, and 25 µg/mL. To a test tube, 100 µL of each concentration of gallic acid standard or each sweet potato extract (10 mg/mL in 100% ethanol) was added, followed by 2 mL of distilled water. After adding 500 µL of Folin and Ciocalteu’s phenol reagent (MP Biomedicals, Santa Ana, CA, USA), 500 µL of a 10% sodium carbonate solution was quickly added, mixed, and placed in the dark for 1 h at room temperature. The absorbance at 760 nm was measured, and the total polyphenol content per 100 g of sample was calculated.
Matsumoto Y., Suto M., Umebara I., Masutomi H, & Ishihara K. (2024). Hydrophobic Components in Light-Yellow Pulp Sweet Potato (Ipomoea batatas (L.) Lam.) Tubers Suppress LPS-Induced Inflammatory Responses in RAW264.7 Cells via Activation of the Nrf2 Pathway. Nutrients, 16(4), 563.
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3

Quantitative Analysis of Polyphenolic Compounds

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Chemical reagents used included
aluminum chloride (99% extrapure, anhydrous, granules), ethyl alcohol
(99% ACS spectroscopic grade), and quercetin hydrate (95%), which
were purchased from Thermo Fisher Scientific. Potassium acetate (certified
ACS crystalline) and sodium carbonate anhydrous (HPLC-grade powder)
were purchased from Fisher Scientific. Folin and Ciocalteu’s
phenol reagent and gallic acid monohydrate (ACS reagent grade) were
purchased from MP Biomedicals (Santa Ana, CA). Sodium acetate (Sigma
Ultra minimum 99.0%) was purchased from Sigma-Aldrich. Conc. HCl,
conc. NaOH, pure ethanol, and 95% ethanol were purchased from Fisher
Scientific. Distilled water was used for all procedures. Ultraviolet/visible
(UV/vis) determination of total concentrations of anthocyanins, flavonoids,
and polyphenols was performed on a Spectronic 10 Genesis spectrophotometer,
as described in the procedures below.
Green B.V., Aroh B., Fiorellino N.M., Ristvey A.G, & Volkis V.V. (2023). Effect of Cultural Management and Plant Age on the Yield, °Brix, and Antioxidant Content of Aronia mitschurinii Grown in Maryland. ACS Omega, 8(4), 4060-4071.
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4

Quantification of Anthocyanins, Flavonoids, and Polyphenols

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Chemical reagents used included
aluminum chloride (99% extra pure, anhydrous, granules), ethyl alcohol
(99% ACS spectroscopic grade), and quercetin hydrate (95%), which
were purchased from Thermo Fisher Scientific. Potassium acetate (certified
ACS Crystalline) and sodium carbonate anhydrous (HPLC grade powder)
were purchased from Fisher Scientific. Folin and Ciocalteu’s
Phenol reagent and gallic acid monohydrate (ACS reagent grade) were
purchased from MP Biomedicals (Santa Ana, CA). Sodium acetate (Sigma
Ultra minimum 99.0%) was purchased from Sigma-Aldrich. Amberlite resins
XAD1180 N, XAD7HP, XAD761, and FPX66 were purchased from Sigma-Aldrich.
Conc. HCl, Conc. NaOH, pure ethanol, and 95% ethanol were purchased
from Fisher Scientific. Distilled water was used for all procedures.
UV/Vis determination of total concentrations of anthocyanins, flavonoids,
and polyphenols was performed on a Spectronic 10 Genesis spectrophotometer,
as described in the procedures below.
Green B.V., Ford T.W., Goldsborrough H., Abdelmotalab M., Ristvey A.G., Sauder D.G, & Volkis V.V. (2022). Extraction of Antioxidants from Aronia mitschurinii Juice Using Macroporous Resins. ACS Omega, 7(34), 29877-29885.
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5

Quantifying Antioxidant Potential in Samples

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The chemical reagents used were: hydrochloric acid solution 32 wt. % in H2O, FCC (Sigma-Aldrich, Merck, Darmstadt, Germany); sodium hydroxide pellets EMPROVE® ESSENTIAL (Merck, Darmstadt, Germany); gallic acid monohydrate ACS reagent ≥98.0% (Sigma-Aldrich, Merck, Darmstadt, Germany); sodium bicarbonate ACS reagent ≥99.7% (Merck, Darmstadt, Germany); methanol ACS EMSURE® (Merck, Darmstadt, Germany); Folin and Ciocalteu's Phenol reagent (MP Biomedicals, Valiant Co. Ltd., China); 2,2-Diphenyl-1-picrylhydrazyl (free radical) 95% (Alfa Aesar, Thermo Fisher Scientific, Kandel, Germany); absolute ethanol ≥99.8%; AnalaR NORMAPUR® ACS (VWR, West Chester, Pennsylvania, United States); formic acid 98% for ACS analysis (PanReac AppliChem, ITW Reagents, Chicago, USA); acetonitrile Optima LC/MS grade (Fisher Chemical, Pittsburgh, PA, USA); and ultrapure water (MilliQ Advantage A10 water purification system, Millipore, Billerica, MA, USA).
Albolafio S., Gil M.I., Allende A., & Xanthakis E. (2020). Potential of Wastewater Valorization after Wet Extraction of Proteins from Faba Bean and Pea Flours. Recent Progress in Materials, 03(02), 1-1.
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