Quanteon flow cytometer

Manufactured by FlowJo

The Quanteon flow cytometer is a laboratory instrument designed for the analysis of cells and other particles. It uses the principles of flow cytometry to measure and analyze multiple characteristics of cells or particles as they pass through a laser beam. The Quanteon provides high-performance data acquisition, analysis, and display capabilities to support a wide range of applications in the life sciences.

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Lab products found in correlation

Anti cd45 clone 30 f11, by BioLegend (1 mentions) Anti f4 80 clone bm8, by BioLegend (1 mentions) Zombie nir viability dye, by BioLegend (1 mentions) Streptavidin apc, by Thermo Fisher Scientific (1 mentions) Anti cd47 clone b6h12, by BioXCell (1 mentions) Anti cd20 apc clone 2h7, by BioLegend (1 mentions) Annexin 5 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd11b clone m1 70, by BioLegend (1 mentions)

2 protocols using quanteon flow cytometer

Quantification of LDLR Expression

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The expression of LDLR upon escalating 4-OI stimulation (75–125 µM) was analyzed using flow cytometry. Briefly, 1.25 × 10⁵ cells were washed with a staining buffer (PBS supplemented with 2% heat-inactivated FCS) and antibodies at a dilution of 1:80 were applied in staining buffer for 60 min at 4 °C in the dark (Mouse Anti-Human-LDLR Antibody, Clone C7, BV421-conjugated (BD Bioscience, Cat# BD744847) or Mouse IgG2 kappa Isotope Control BV421-conjugated, both from (BD Bioscience, Cat# BD569376)). Cells were then washed twice, and fixed using 1% paraformaldehyde, pH 7.4 (Morphisto). The fluorescence intensity was measured with a NovoCyte Quanteon flow cytometer and analyzed using FlowJo software (v10). Samples stained with the isotype control of the matched antibody were used as a control, and the background MFI obtained was subtracted from the MFI of the separate samples. The gating strategy on LDLR+ cells is described in Fig. S8a.

Kurmasheva N., Said A., Wong B., Kinderman P., Han X., Rahimic A.H., Kress A., Carter-Timofte M.E., Holm E., van der Horst D., Kollmann C.F., Liu Z., Wang C., Hoang H.D., Kovalenko E., Chrysopoulou M., Twayana K.S., Ottosen R.N., Svenningsen E.B., Begnini F., Kiib A.E., Kromm F.E., Weiss H.J., Di Carlo D., Muscolini M., Higgins M., van der Heijden M., Bardoul A., Tong T., Ozsvar A., Hou W.H., Schack V.R., Holm C.K., Zheng Y., Ruzek M., Kalucka J., de la Vega L., Elgaher W.A., Korshoej A.R., Lin R., Hiscott J., Poulsen T.B., O’Neill L.A., Roy D.G., Rinschen M.M., van Montfoort N., Diallo J.S., Farin H.F., Alain T, & Olagnier D. (2024). Octyl itaconate enhances VSVΔ51 oncolytic virotherapy by multitarget inhibition of antiviral and inflammatory pathways. Nature Communications, 15, 4096.

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Flow Cytometric Profiling of Ramos Cells

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Ramos cells were analysed by flow cytometry using the following antibodies: anti-CD20-APC (clone 2H7, Biolegend), anti-CD2-APC (clone REA972, Mitenyi Biotech), anti-CD47 (clone B6.H12, BioXCell), and anti-Calreticulin-DyLight-488 (FMC 75, Enzo Life Sciences), and with Annexin-V FITC (A13199, Thermo Fisher), or with biotinylated Maackia amurensis lectin II (MAL-II) (B-1265, VectorLabs) followed by streptavidin-APC (Thermo Fisher). Sialidase (neuraminidase from Vibrio cholerae, Sigma 11080725001) treatment was performed by incubating cells with 30 nM enzyme in dPBS at a cell concentration of 1 × 10⁶ ml⁻¹ for 1 h at 37 °C. In Extended Data Fig. 6j, single-cell suspensions were prepared from diced tumours and fixed in paraformaldehyde as described previously. Cells were stained with Zombie-NIR viability dye (BioLegend), anti-CD45 (clone 30-F11, BioLegend), anti-F4/80 (clone BM8, BioLegend) and anti-CD11b (clone M1/70, BioLegend) and analysed using an Acea Novocyte Quanteon flow cytometer and FlowJo software (version 10.6.1). Live CD45⁺ cells were counted using gates that separated the CD45⁻ and CD45⁺ populations, and macrophages were counted using a single gate applied equally to all samples.

Kamber R.A., Nishiga Y., Morton B., Banuelos A.M., Barkal A.A., Vences-Catalán F., Gu M., Fernandez D., Seoane J.A., Yao D., Liu K., Lin S., Spees K., Curtis C., Jerby-Arnon L., Weissman I.L., Sage J, & Bassik M.C. (2021). Inter-cellular CRISPR screens reveal regulators of cancer cell phagocytosis. Nature, 597(7877), 549-554.

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