Anti actin antibody sc 1616

Manufactured by Santa Cruz Biotechnology

The Anti-actin antibody (sc-1616) is a primary antibody that specifically recognizes actin, a ubiquitous cytoskeletal protein found in all eukaryotic cells. This antibody can be used to detect and quantify actin levels in various experimental applications.

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Lab products found in correlation

Enhanced chemiluminescence system, by GE Healthcare (1 mentions) Complete mini protease inhibitor cocktail tablet, by Roche (1 mentions) Aspirin, by Merck Group (1 mentions) Celecoxib, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Ldh cytotoxicity kit, by Thermo Fisher Scientific (1 mentions) High glucose dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Dispase, by BD (1 mentions) Waymouth s medium, by Thermo Fisher Scientific (1 mentions) Hrp conjugated donkey anti goat igg, by Santa Cruz Biotechnology (1 mentions) C terminal gfp tagged open reading frame clone of full length human vegfr2, by OriGene (1 mentions) Ham s f10, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Percoll, by Merck Group (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Trypsin, by Merck Group (1 mentions) Dnase, by Merck Group (1 mentions) Nitrocellulose membrane, by Merck Group (1 mentions) Ab128926, by Abcam (1 mentions) Ecl plus, by GE Healthcare (1 mentions)

5 protocols using anti actin antibody sc 1616

Western Blot Analysis of NDR1/2 Phosphorylation

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The following rabbit polyclonal antibodies were used for Western blotting: total NDR1 and NDR2 [7 (link)]; phospho-T444/T442 [65 (link)]. The anti-actin antibody (sc-1616) was obtained from Santa Cruz, the HSC70 antibody (Clone 1B5) from Stressgen. Western blotting analysis was done as described previously [7 (link)].

Schmitz-Rohmer D., Probst S., Yang Z.Z., Laurent F., Stadler M.B., Zuniga A., Zeller R., Hynx D., Hemmings B.A, & Hergovich A. (2015). NDR Kinases Are Essential for Somitogenesis and Cardiac Looping during Mouse Embryonic Development. PLoS ONE, 10(8), e0136566.

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Quantifying Actin Isoforms in Tumor Cells

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Proteins were extracted from tumors or CNDT2.5 cells using Cytobuster™ protein extraction reagent (Novagen) supplemented with Complete mini protease inhibitor cocktail tablets (Roche Diagnostics). Analysis of ACTG2 in tumor tissue was done using a primary antibody; anti-actin gamma2 (NB100-91649). Anti-Actin antibody (sc 1616, Santa Cruz) or coomassie blue was used as loading controls, and for verification of transfection results a mouse monoclonal anti-DDK antibody (TA50011, Origene) was used. After incubation with the appropriate secondary antibody, bands were visualized using the enhanced chemiluminescence system (GE Healthcare).

Edfeldt K., Hellman P., Westin G, & Stalberg P. (2016). A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis. BMC Endocrine Disorders, 16, 19.

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Endothelial Cell Functional Assays

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Aspirin, celecoxib, trypsin, DNAse and Percoll were purchased from Sigma-Aldrich (St. Louis, MO). Dispase was purchased from B&D Bioscience (San Jose, CA). 5-(4-Chlorophenyl)-1-(4-methoxyphenyl)-3-trifluoromethylpyrazole (sc-560) was purchased from EMD Millipore (Billerica, MA). The ELISA Kit for human sVEGFR1 and anti-Flt1 antibody (AF321) were obtained from R&D Systems (Minneapolis, MN). Cleaved poly ADP-ribose polymerase (PARP) antibody, anti-caspase-3 and HRP-conjugated goat anti-rabbit IgG were purchased from Cell Signaling Technology (Danvers, MA). Anti-actin antibody (sc-1616) and HRP-conjugated donkey anti-goat IgG were from Santa Cruz Biotechnology (Santa Cruz, CA). The LDH cytotoxicity kit was purchased from Thermo-Fisher Scientific (Rockford, IL). Ham's F-10, Waymouth’s medium, high Glucose Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were from Life Technologies (Grand Island, NY). C-terminal GFP-tagged open reading frame (ORF) clone of full length human VEGFR2 was purchased from OriGene (Rockville, MD). Recombinant adenovirus expressing FLT, sFLT1-i13, sFLT1-e15a and control virus were generated by the vector Core Facility at the University of Iowa and has been previously described (4 (link), 6 (link)).

Li C., Raikwar N.S., Santillan M.K., Santillan D.A, & Thomas C.P. (2015). Aspirin inhibits expression of sFLT1 from human cytotrophoblasts induced by hypoxia, via cyclo-oxygenase 1. Placenta, 36(4), 446-453.

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Western Blot Analysis of HGFA Inhibitor 2

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Equal amounts of protein were separated by sodium dodecyl sulphate‐polyacrylamide electrophoresis and transferred to nitrocellulose membranes (Millipore). Detection was carried out with the chemiluminescent substrate using an ECL Plus (GE‐Healthcare). Monoclonal anti‐HGFA Inhibitor 2 (HAI‐2) antibody (ab128926) was purchased from Abcam or anti‐Actin antibody (sc‐1616) from Santa Cruz Biotechnology. Quantitative analyses of the optical intensities of protein bands were determined with Un‐Scan‐It Gel 6.1 (Silk Scientific Inc.) and normalized by actin.

Roversi F.M., Cury N.M., Lopes M.R., Ferro K.P., Machado‐Neto J.A., Alvarez M.C., dos Santos G.P., Giardini Rosa R., Longhini A.L., Duarte A.D., Pericole F.V., Favaro P., Yunes J.A, & Saad S.T. (2018). Up‐regulation of SPINT2/HAI‐2 by Azacytidine in bone marrow mesenchymal stromal cells affects leukemic stem cell survival and adhesion. Journal of Cellular and Molecular Medicine, 23(2), 1562-1571.

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Quantitative Western Blot Analysis of Tagged Proteins

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Whole-cell extracts were run on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Western blot analysis was performed by standard methods using a mouse anti-GFP antibody (sc-9996, Santa Cruz Biotechnology) for the detection of GFP-tagged proteins, an HRP-conjugated anti-mouse IgG antibody (A9044, Sigma) for the detection of TAP-tagged proteins, an HRP-conjugated anti-HA antibody (sc-7392, Santa Cruz Biotechnology) for the detection of HA-tagged proteins, and a mouse anti-Myc antibody (sc-40, Santa Cruz Biotechnology) for the detection of Myc-tagged proteins. Actin and hexokinase were used as loading controls and were detected by an anti-actin antibody (sc-1616, Santa Cruz Biotechnology) and an anti-hexokinase antibody (H2035-02, United States Biological), respectively. Each western blotting experiment was performed at least three independent times. Images were captured using a luminescent image analyzer LAS-3000 (Fujifilm) and quantification of captured images was performed using Multi Gauge V3.0 software (Fujifilm).

Ha C.W., Kim K., Chang Y.J., Kim B, & Huh W.K. (2014). The β-1,3-glucanosyltransferase Gas1 regulates Sir2-mediated rDNA stability in Saccharomyces cerevisiae. Nucleic Acids Research, 42(13), 8486-8499.

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