Fluorescence anisotropy was measured using the same 40 bp dsDNA as the one used in ATPase assay, but with one strand modified at the 3′ end with fluorescein. Measurements were recorded using a Synergy Neo Hybrid Multi‐Mode Microplate reader (BioTek) with the appropriate filters in black 96‐well flat bottom plates at 25°C. Buffer conditions were identical to those used in ATPase activity measurements. Anisotropy measurements were exported from the BioTek Synergy Neo software and subsequently fit to a binding polynomial using non‐linear regression in GraphPad Prism 8.
Synergy neo hybrid multi mode microplate reader
Manufactured by Agilent Technologies
The Synergy Neo Hybrid Multi‐Mode Microplate Reader is a versatile laboratory instrument designed for various detection modes, including absorbance, fluorescence, luminescence, and time-resolved fluorescence. It is capable of performing diverse assays and applications within a microplate format.
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Lab products found in correlation
2 protocols using synergy neo hybrid multi mode microplate reader
1
Fluorescence Anisotropy Protocol for DNA Binding
2
Fluorescence Anisotropy Assay for dsDNA
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