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Pdgf dd

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PDGF-DD is a recombinant human platelet-derived growth factor subunit D. It is a dimeric protein that belongs to the PDGF family of growth factors. PDGF-DD is involved in the regulation of cell growth and division.

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Lab products found in correlation

Incucyte live cell analysis system, by Sartorius (2 mentions) Crenolanib, by Selleck Chemicals (1 mentions) Igf 1, by Thermo Fisher Scientific (1 mentions) Sdf 1α, by R&D Systems (1 mentions) Pdgf aa, by R&D Systems (1 mentions) Bmp 2, by Medtronic (1 mentions) Pdgf ab, by R&D Systems (1 mentions) Pdgf cc, by R&D Systems (1 mentions) Pdgf aa, by Selleck Chemicals (1 mentions) Pdgf bb, by R&D Systems (1 mentions) Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions) Tgf β3, by Miltenyi Biotec (1 mentions)

5 protocols using pdgf dd

1

PDGF-DD Stimulation of NK Cells

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IL-2 cultured human NK cells were incubated with 250 ng/ml PDGF-DD (R&D Systems) in 24-well plates for 16h in the presence of 1 μg/ml IgG1 (NKDD+IgG sup) or blocking anti-NKp44 3.43.14 mAb (NKDD+α-NKp44 sup). After 16h, supernatants were pooled, centrifuged and filtered to remove cellular debris, and then stored at −80°C until further use. B16-pMX and B16-PDGFD stable cell-lines were cultured in 6-well plates for 72h. After 72h, supernatants were centrifuge, filtered and then stored at +4°C until further use.
Barrow A.D., Edeling M.A., Trifonov V., Luo J., Goyal P., Bohl B., Bando J., Kim A.H., Walker J., Andahazy M., Bugatti M., Melocchi L., Vermi W., Fremont D.H., Cox S., Cella M., Schmedt C, & Colonna M. (2017). Natural Killer cells control tumor growth by sensing a growth factor. Cell, 172(3), 534-548.e19.
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2

HASMC Migration Modulation by PDGF-DD

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Human aortic smooth muscle cells (HASMC) were plated at 8000 cells per well in upper chamber of 96-well transmigration plates from Incucyte (cat # 4648) and left to attach and spread for 30 hours. Cells were then washed with serum free media 2 times and incubated in serum free media for 12 hours. PDGF-DD (R&D systems, #1159-SB) was solubilized in 4mM HCl (Vehicle). PDGF-DD (17.85nM) was preincubated for 20 mins with or without anti-PDGF-DD antibody 25E17 (35.7nM) in serum free medium. PDGF-DD with or without antibody was added to the lower chamber of transmigration plates. Vehicle was added in control wells. Live cell migration was measured with the Incucyte Live-Cell Analysis system (Essen Bioscience). All data is expressed as the mean ± standard error of the mean (SEM). Statistical analysis performed using a repeated measures one-way ANOVA with Tukey correction.
Kim H.J., Cheng P., Travisano S., Weldy C., Monteiro J.P., Kundu R., Nguyen T., Sharma D., Shi H., Lin Y., Liu B., Haldar S., Jackson S, & Quertermous T. (2023). Molecular mechanisms of coronary artery disease risk at the PDGFD locus. bioRxiv.
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3

Quantification of PDGF-DD in TGF-β-treated MRC5 cells

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An ELISA kit was obtained to measure concentrations of PDGF-DD (R&D systems) in supernatants of MRC5 cells cultured in the absence or presence of TGFβ (4 ng/ml) for 24 h. The detection limit of this assay was 1.67 pg/ml.
Charni Chaabane S., Coomans de Brachène A., Essaghir A., Velghe A., Lo Re S., Stockis J., Lucas S., Khachigian L.M., Huaux F, & Demoulin J.B. (2014). PDGF-D Expression Is Down-Regulated by TGFβ in Fibroblasts. PLoS ONE, 9(10), e108656.
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4

PDGF-DD Modulates HASMC Proliferation

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Human aortic smooth muscle cells (HASMC) were plated at 5000 cells per well in 96-well plates and left to attach and spread for 30 hours. Cells were then washed with serum free media 2 times and incubated in serum free media for 6 hours. PDGF-DD (R&D systems, #1159-SB) was solubilized in 4mM HCl (Vehicle). PDGF-DD (17.85nM) was preincubated for 20 mins with or without anti-PDGF-DD antibody 25E17 (35.7nM) in serum free medium. Cells were treated with PDGF-DD with or without antibody in serum free media. Vehicle was added in control wells. Live cell proliferation was measured with the Incucyte Live-Cell Analysis system (Essen Bioscience). All data is expressed as the mean ± standard error of the mean (SEM). Statistical analysis performed using a repeated measures one-way ANOVA with Tukey correction.
Kim H.J., Cheng P., Travisano S., Weldy C., Monteiro J.P., Kundu R., Nguyen T., Sharma D., Shi H., Lin Y., Liu B., Haldar S., Jackson S, & Quertermous T. (2023). Molecular mechanisms of coronary artery disease risk at the PDGFD locus. bioRxiv.
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5

Chemotactic migration of cells

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We used recombinant human bone morphogenetic protein-2 (BMP-2; Medtronic, Minneapolis, MN, USA), hepatocyte growth factor (HGF; Peprotech, Rocky Hill, NJ, USA), insulin-like growth factor-1 (IGF-1; Peprotech), platelet-derived growth factor-AA (PDGF-AA; R&D Systems), PDGF-AB (R&D Systems), PDGF-BB (R&D Systems), PDGF-CC (R&D Systems), PDGF-DD (R&D Systems), stromal cell-derived factor-1α (SDF-1α; R&D Systems), transforming growth factor-β3 (TGF-β3; Miltenyi Biotec, Bergisch Gladbach, Germany). The medium containing 0.5% FBS in the lower chamber was supplemented with 100 ng/mL BMP-2, 100 ng/mL HGF, 100 ng/mL IGF-1, 10 ng/mL PDGF-BB, 100 ng/mL SDF-1α, or 10 ng/mL TGF-β3. After 48 h incubation, migrated cells were counted. The PDGF isoforms were compared by adding PDGF-AA, AB, BB, CC, or DD to the lower chamber at 100 ng/mL. Crenolanib (Selleck Chemicals, Houston, TX, USA), an inhibitor of PDGF receptor (PDGFR) α and β, was added to both the upper and lower chambers during the migration assay.
Endo K., Horiuchi K., Katano H., Ozeki N., Sakamaki Y., Koga H, & Sekiya I. (2021). Intra-articular Injection of PDGF-BB Explored in a Novel in Vitro Model Mobilizes Mesenchymal Stem Cells From the Synovium Into Synovial Fluid in Rats. Stem cell reviews and reports, 17(5).
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