All data were collected at the St. Augustine Alligator Farm Zoological Park located in Saint Augustine, Florida, USA between March and June 2013. The subjects were 43 sexually mature American alligators. Most of the animals (13 females and 29 males) were kept together in an outdoor enclosure (roughly 557 m2) with ponds, an island, and several sand beaches (two adult albino alligators, a male and a female, were singly housed in roofed enclosures for protection against sunburn). The two largest males in the group were wild caught; all other animals were hatched and raised in captivity. All alligators were accustomed to daily interactions with their keepers and roughly half would not retreat when approached and touched. Each study alligator had an implanted microchip under its nuchal plates for identification. In addition, the animals could be individually discriminated by specific characteristics such as tooth position, scars, head shape, coloration, osteoderm and scale patterns. Previous to data recording, a profile of each subject was created. Its chip was read and its particularities photographed from different angles (Canon Powershot SX50).
Powershot sx50
Manufactured by Canon
The Canon Powershot SX50 is a compact digital camera with a high-powered 50x optical zoom lens. It features a 12.1-megapixel CMOS sensor and can record 1080p HD video. The Powershot SX50 provides advanced photographic capabilities in a portable form factor.
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Lab products found in correlation
3 protocols using powershot sx50
1
Captive American Alligator Behavioral Study
2
Entomological Specimen Dissection and Imaging
3
Entomological Specimen Dissection and Imaging
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Standard entomological techniques were used for dissection (Robbins, 1991 ): the distal part of the abdomen was broken off, soaked for 40 minutes (or until cleared) in 10% KOH at 60°C, dissected, and subsequently stored in a small glycerol-filled vial on the pin under the specimen. Genitalia and wing venation terminology follow Steinhauser (1981) , except that cucullus is called harpe in this work, and we use the term “cheek” in the sense of Evans (1951) to refer to the dense clump of hair-like scales immediately below the eye. Photographs of specimens and habitat were taken by RJG with a Canon Powershot SX50 camera; dissected genitalia were photographed by NVG in glycerol with a Nikon D200 camera without the lens and through microscope at about 1x magnification. Genitalia photographs were taken in several focus slices and stacked in Photoshop to increase depth of field. Images were assembled and edited in Photoshop CS5.1. DNA sequencing and analysis techniques were the same as reported by Zhang et al. (2019) .
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