Ab52302

Manufactured by Abcam

Sourced in United Kingdom

Ab52302 is a laboratory equipment product. It is a device designed for scientific research purposes. The core function of this product is to facilitate specific laboratory procedures and experiments. No further details about the intended use or performance of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Ab8925, by Abcam (1 mentions) Cd10 56c6, by Agilent Technologies (1 mentions) P50 sc 8414, by Santa Cruz Biotechnology (1 mentions) Mum1 mum1p, by Agilent Technologies (1 mentions)

2 protocols using ab52302

Immunohistochemical Analysis of Activated NOTCH Signaling in HCC

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We used tissue microarrays for human primary and metastatic HCCs (SuperBioChips Laboratories, Seoul, Korea). Immunohistochemical staining was performed as previously described (8 (link)), using primary antibodies against human activated NOTCH2 (ab52302), activated NOTCH1 (ab8925) (both from Abcam, Cambridge, UK), α-fetoprotein (AFP), cytokeratin 19 (CK19) (both from Dako, Glostrup, Denmark), and EpCAM (ab187270; Abcam). The anti-NOTCH antibodies have been shown to react only with the activated forms of the intracellular domain after cleavage by γ-secretase (1 (link)). Clinical staging or histopathological grading of differentiation of the primary HCCs were performed according to the American Joint Committee on Cancer (AJCC) Cancer Staging Manual (9 ), or the General Rules for the Clinical and Pathological Study of Primary Liver Cancer (10 ), respectively. For quantification of the nuclear/cytoplasmic (N/C) ratio and nuclear density, the HCC tissues on the tissue microarray were photographed and analyzed using ImageJ software (http://rsb.info.nih.gov/ij/).

HAYASHI Y., OSANAI M, & LEE G.H. (2015). NOTCH2 signaling confers immature morphology and aggressiveness in human hepatocellular carcinoma cells. Oncology Reports, 34(4), 1650-1658.

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Immunohistochemical Staining Protocol for DLBCL

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The immunohistochemical staining of DLBCLs was performed according to the protocol [18] (link). The immunohistochemistrical antibodies were listed as follows: CD10 (56C6, Dako), Bcl6 (M7211, Dako), MUM1 (MUM1p, Dako), Ki67 (MB1, Dako), P50 (sc-8414, Santa Cruz), P65 (#4764, Cell Signaling) and Notch2 (ab52302, Abcam). For Ki67 [26] (link) and Notch2 [27] (link), cases were considered to be positive when 25% or 20% of lymphoma cells were stained, respectively. For the rest antibodies, we defined 30% as the cut-off [28] (link), [29] (link).

Zhang X., Shi Y., Weng Y., Lai Q., Luo T., Zhao J., Ren G., Li W., Pan H., Ke Y., Zhang W., He Q., Wang Q, & Zhou R. (2014). The Truncate Mutation of Notch2 Enhances Cell Proliferation through Activating the NF-κB Signal Pathway in the Diffuse Large B-Cell Lymphomas. PLoS ONE, 9(10), e108747.

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