LNCaP and C4-2 cells were obtained from the American Type Tissue Collection (ATCC) (Rockville, MD). Cells were grown in RPMI 1640 media (Corning, Manassas, VA) containing either 10% fetal bovine serum (Gemini Bio-Products, West Sacramento, CA) or 10% charcoal stripped fetal bovine serum (HyClone, Logan, UT and Sigma, St. Louis, MO) and supplemented with Ciprofloxacin Hydrochloride 5μg/ml (US Biological, Swampscott, MA), and Gentamicin 50 μg/ml (Quality Biological Inc., Gaithersburg, MD). The cells were passaged in uncoated filter top polystyrene flasks (Corning) and were maintained at 37°C in 5% CO2 in humidified air. Cells were typically split at 80% confluency using 0.05% trypsin (cat # 25-052-CI; Corning) in calcium-free Hank's Buffered Salt Solution (Corning).
Hank s buffered salt solution
Manufactured by Corning
Hank's Buffered Salt Solution is a commonly used buffer solution in cell culture and biological research. It is a balanced salt solution that provides a physiologically relevant environment for cells and tissues. The solution contains a mixture of inorganic salts, including sodium chloride, potassium chloride, calcium chloride, and magnesium chloride, as well as a buffering agent to maintain a stable pH.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 media, by Corning (1 mentions)
Ciprofloxacin hydrochloride, by US Biological (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Trypsin, by Corning (1 mentions)
Gentamicin, by Quality Biological (1 mentions)
40 μm filter, by Avantor (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Collagenase, by Merck Group (1 mentions)
Poly d lysine collagen, by Merck Group (1 mentions)
Pasteur pipette, by Avantor (1 mentions)
Hepes, by Merck Group (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Neurobasal a medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Papain, by Worthington (1 mentions)
2 protocols using hank s buffered salt solution
1
Cell Culture of Prostate Cancer Lines
2
Dorsal Root Ganglion Neuron Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For each tissue preparation, two age- and sex-matched mice were killed by live decapitation, and cervical through lumbar DRG were removed and pooled together. DRG were incubated in papain (45 U, Worthington) for 20 min at 37°C, 5% CO2. DRG were then rinsed and incubated in collagenase (1.5 mg/mL, Sigma-Aldrich) for 20 min. Both enzyme solutions were made up in Ca2+- and Mg2+-free Hanks’ buffered salt solution (Corning) with 10 mm Hepes (Sigma-Aldrich). DRG were manually triturated with fire-polished Pasteur pipettes (VWR) to dissociate neurons, passed through a 40-μm filter (VWR), and plated onto poly-d -lysine/collagen (Sigma-Aldrich)-coated 12-mm glass coverslips (Thermo Fisher Scientific). Neurons were maintained in culture for 2 d in Neurobasal A medium (Invitrogen) supplemented with 100 U/mL penicillin/streptomycin (Corning), 2 mm GlutaMAX (Life Technologies), 2% B27 (Gibco), and 5% fetal bovine serum (Gibco).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!