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Supersignal chemiluminescent substrates signal

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SuperSignal chemiluminescent substrates are used to detect the presence and quantity of proteins in Western blot analysis. These substrates produce a luminescent signal when they react with the enzyme-labeled antibodies bound to the target proteins, allowing for the visualization and quantification of the proteins of interest.

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Lab products found in correlation

Ai600 chemiluminescent imager, by GE Healthcare (2 mentions) Rabbit anti pan actin, by Cell Signaling Technology (1 mentions) Pa1 901, by Cell Signaling Technology (1 mentions) Rabbit pan actin, by Cell Signaling Technology (1 mentions)

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SuperSignal Chemiluminescent Substrate SuperSignal substrates Chemiluminescent substrate

2 protocols using supersignal chemiluminescent substrates signal

1

Western Blot Analysis of DRG Neuron Proteins

Cited 1 time
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DRG neurons transduced with Sarm1, IP3R1, or control shRNA for 6–7 d were lysed with nonionic detergent. Lysates were separated by 3–8% Tris-Acetate SDS-PAGE for IP3R1 and by 4–12% Bis-Tris SDS-Page for Sarm1 (Thermo Fisher Scientific) and probed with mouse anti-Sarm1 (1:10,000; Chen et al., 2011 (link)), rabbit anti-IP3R1 (1:1,000; Thermo Fisher Scientific; PA1-901), and rabbit anti–pan-actin (1:1,000; Cell Signaling; 8456S). Bands were visualized with secondary antibodies conjugated to HRP (1:10,000; Bio-Rad; 1721019, 1706516) and SuperSignal chemiluminescent substrates signal (Thermo Fisher Scientific). Blots were imaged using the AI600 Chemiluminescent Imager (GE Healthcare).
Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C.Y., Hsueh Y.P., Bergholz J.S., Jiang T., Zhao J.J., Zhu J., Ko K.W., Milbrandt J., DiAntonio A, & Segal R.A. (2021). Sarm1 activation produces cADPR to increase intra-axonal Ca++ and promote axon degeneration in PIPN. The Journal of Cell Biology, 221(2), e202106080.
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2

Quantifying IP3R1 Expression in DRG Neurons

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For assessing IP 3 R1 level, DRG neurons transduced with IP 3 R1 or control shRNA for 6-7 days were lysed with non-ionic detergent. Lysates were separated by 3%-8% Tris-Acetate SDS-Page (Thermo fisher) and probed against rabbit IP 3 R1 (1:1000; Thermo Fisher) and rabbit pan-actin (1:1000; Cell signaling). Bands were visualized with secondary antibodies conjugated to HRP (1:10,000; Bio-Rad) and SuperSignal chemiluminescent substrates signal (Thermo Fisher). Blots were imaged using AI600 Chemiluminescent Imager (GE Healthcare).
Li Y., Pazyra‐Murphy M.F., Avizonis D., Russo M.D.S.T., Tang S., Bergholz J.S., Jiang T., Zhao J.J., Zhu J., Ko K.W., Milbrandt J., DiAntonio A., & Segal R.A. (2021). Activation of Sarm1 produces cADPR to increase intra-axonal calcium and promote axon degeneration in CIPN.
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