Log In Sign up
The largest database of trusted experimental protocols

Amicon ultra 4 50k mwco centrifugation filter

Manufactured by Merck Group
Visit Supplier

The Amicon Ultra-4 50K MWCO centrifugation filter is a laboratory device designed for the concentration and purification of macromolecules. It utilizes a centrifugation process to separate molecules based on their molecular weight, allowing for the retention of target analytes while removing smaller components from the sample.

Automatically generated - may contain errors

Lab products found in correlation

Silver staining, by Thermo Fisher Scientific (2 mentions) Galanthis nivalis lectin, by Merck Group (2 mentions) Sypro ruby protein staining, by Lonza (2 mentions) Bn page, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Amicon Ultra-4 50K filter Amicon Ultra-4 50K Amicon Ultra 50K Amicon centrifugation filters Amicon Ultra filters Amicon Ultra-4 Centrifugation filters MWCO) filters Amicon filters Amicon Ultra

2 protocols using amicon ultra 4 50k mwco centrifugation filter

1

Salipro-HIV-Spike Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Salipro-HIV-spikes were purified using Galanthis Nivalis lectin (Sigma-Aldrich) affinity chromatography. The lectin binds to high mannose sugar moieties on the spike protein. The column was washed with 50 column volumes HN buffer followed by elution of the Salipro-HIV-spike particles using 0.3M methyl-αD-mannopyranoside. The methyl-αD-mannopyranoside was removed from the sample using a spin SEC column (Zeba Spin Desalting Columns, 7K MWCO) and changed to HN buffer. The Salipro-HIV-spikes were concentrated by ultra filtration using an Amicon Ultra-4 50K MWCO centrifugation filter (Millipore) and sample purity was assayed with SDS-PAGE using Sypro Ruby protein staining (Lonza group Ltd) and BN-PAGE using silver staining (Thermo Scientific).
Frauenfeld J., Löving R., Armache J.P., Sonnen A., Guettou F., Moberg P., Zhu L., Jegerschöld C., Flayhan A., Briggs J.A., Garoff H., Löw C., Cheng Y, & Nordlund P. (2016). A novel lipoprotein nanoparticle system for membrane proteins. Nature methods, 13(4), 345-351.
+ Open protocol
+ Expand
2

Salipro-HIV-Spike Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Salipro-HIV-spikes were purified using Galanthis Nivalis lectin (Sigma-Aldrich) affinity chromatography. The lectin binds to high mannose sugar moieties on the spike protein. The column was washed with 50 column volumes HN buffer followed by elution of the Salipro-HIV-spike particles using 0.3M methyl-αD-mannopyranoside. The methyl-αD-mannopyranoside was removed from the sample using a spin SEC column (Zeba Spin Desalting Columns, 7K MWCO) and changed to HN buffer. The Salipro-HIV-spikes were concentrated by ultra filtration using an Amicon Ultra-4 50K MWCO centrifugation filter (Millipore) and sample purity was assayed with SDS-PAGE using Sypro Ruby protein staining (Lonza group Ltd) and BN-PAGE using silver staining (Thermo Scientific).
Frauenfeld J., Löving R., Armache J.P., Sonnen A., Guettou F., Moberg P., Zhu L., Jegerschöld C., Flayhan A., Briggs J.A., Garoff H., Löw C., Cheng Y, & Nordlund P. (2016). A novel lipoprotein nanoparticle system for membrane proteins. Nature methods, 13(4), 345-351.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!