Live dead baclight kittm

Fragmented L. pulmonaria samples from the herbarium of the University of Graz (GZU) were stained with the LIVE/DEAD® Baclight kit^TM (Molecular Probes). The imaging was performed with a confocal laser scanning microscope (Leica TCS SPE confocal microscope, Leica Microsystems). Excitation wavelengths of 488 and 532 nm were used for the SYTO® 9 and propidium iodide fluorescent dye respectively. The light emission was detected in the range of 496–560 nm for SYTO® 9 and 600–680 nm for propidium iodide. Settings for photomultiplier gain and offset were adjusted to obtain an optimal signal/noise ratio. The confocal stacks were merged to obtain a maximum projection of all channels.

Fragmented eggshells from treated and control samples were stained with the LIVE/DEAD® Baclight kitTM (Molecular Probes). The imaging was performed with a confocal laser scanning microscope (Leica TCS SPE confocal microscope, Leica Microsystems). Excitation wavelengths of 488 and 532 nm were used for the SYTO® 9 and propidium iodide fluorescent dye respectively. The light emission was detected in the range of 496–560 nm for SYTO® 9 and 600–680 nm for propidium iodide. Settings for photomultiplier gain and offset were adjusted to obtain an optimal signal/noise ratio. The confocal stacks were merged to obtain a maxium projection of all channels.