Log In Sign up
The largest database of trusted experimental protocols

Cell prep station

Manufactured by NanoString
Visit Supplier
Sourced in United States

The Cell Prep Station is a specialized lab equipment designed for sample preparation. It provides a controlled environment for various cell-based workflows, enabling efficient and reproducible sample processing.

Automatically generated - may contain errors

Lab products found in correlation

Ncounter human immunology kit panel, by NanoString (2 mentions) Digital analyzer, by NanoString (2 mentions) Purelink rna mini kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using cell prep station

1

Immunoinflammatory Transcriptome Analysis of OECs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoinflammatory transcriptome analysis of OECs exposed to the oral pathogen Pg or the oral commensal S. gordonii [MOI-1:100 for 24h] was performed using a set of 520 genes defined by the nCounter Human Immunology Kit panel (Nanostring, Seattle, WA). Briefly, after bacterial exposure, total mRNA was isolated using the PureLink RNA Mini kit (Life Technologies, NY, USA). Further, 100ng of RNA were hybridized with the reporter code set beads for a final volume of 30μl at 65°C for 12 hours. Finally, the samples were processed on the Nanostring Cell Prep Station and data collected using the Nanostring Digital Analyzer version (Nanostring Technologies, Seattle, WA, USA).
Al-Attar A., Alimova Y., Kirakodu S., Kozal A., Novak M.J., Stromberg A.J., Orraca L., Gonzalez-Martinez J., Martinez M., Ebersole J.L, & Gonzalez O.A. (2018). Activation of Notch-1 in oral epithelial cells by P. gingivalis triggers the expression of the antimicrobial protein PLA2-IIA. Mucosal immunology, 11(4), 1047-1059.
+ Open protocol
+ Expand
2

Immunoinflammatory Transcriptome Analysis of OECs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoinflammatory transcriptome analysis of OECs exposed to the oral pathogen Pg or the oral commensal S. gordonii [MOI-1:100 for 24h] was performed using a set of 520 genes defined by the nCounter Human Immunology Kit panel (Nanostring, Seattle, WA). Briefly, after bacterial exposure, total mRNA was isolated using the PureLink RNA Mini kit (Life Technologies, NY, USA). Further, 100ng of RNA were hybridized with the reporter code set beads for a final volume of 30μl at 65°C for 12 hours. Finally, the samples were processed on the Nanostring Cell Prep Station and data collected using the Nanostring Digital Analyzer version (Nanostring Technologies, Seattle, WA, USA).
Al-Attar A., Alimova Y., Kirakodu S., Kozal A., Novak M.J., Stromberg A.J., Orraca L., Gonzalez-Martinez J., Martinez M., Ebersole J.L, & Gonzalez O.A. (2018). Activation of Notch-1 in oral epithelial cells by P. gingivalis triggers the expression of the antimicrobial protein PLA2-IIA. Mucosal immunology, 11(4), 1047-1059.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!