Qiaamp dna blood midi mini kit

Genomic DNA was extracted from 2 mL of cord blood or whole blood or 15 mL of amniotic fluid using the QIAamp DNA Blood Midi/Mini Kit (Qiagen GmbH), following the manufacturer’s instructions.

Fetal samples were obtained via amniocentesis (gestational age <25 weeks) and cord blood sampling (>25 weeks or oligohydramnios). Using a QIAamp DNA Blood Midi/Mini Kit (Qiagen GmbH, Dusseldorf, Germany), fetal genomic DNA samples were obtained from 15 mL of amniotic fluid or 2 mL of cord blood, following the manufacturer’s instructions. Parental blood samples for trio-ES analysis were collected with informed consent.

QIAamp Circulating Nucleic Acid kit (Qiagen, Hilden, Germany) was used according to manufacturer’s protocol to isolate cfDNA from patient serum samples. Genomic DNA from tumor formalin-fixed paraffin-embedded (FFPE) sections, fresh frozen samples (FF) and blood samples were isolated with a High Pure FFPET DNA Isolation Kit (Roche Diagnostics, Indianapolis, IN, USA) and QIAamp DNA Blood Midi/Mini Kit (Qiagen) according to manufacturer’s protocol. Quality and concentration of DNA was assessed with Nanodrop ND-1000 (Thermo Fischer Scientific, Waltham, MA, USA) and Qubit 2.0 with a Qubit dsDNA BR Assay Kit (Invitrogen, Carlsbad, CA, USA). Quality and fragmentation of cfDNA was further analyzed with the TapeStation 4200 electrophoresis system with a D5000 High Sensitivity ScreenTape Assay (Agilent Technologies, Santa Clara, CA, USA) to identify possible gDNA contaminations (Figure S1).