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Metal grid floor

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The Metal Grid Floor is a durable and sturdy laboratory equipment designed to provide a raised, perforated surface for various applications. Its core function is to offer a stable and ventilated platform for housing or supporting laboratory samples, equipment, or materials.

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Lab products found in correlation

Standard conditioning chamber, by Harvard Apparatus (4 mentions)

6 protocols using metal grid floor

1

Pavlovian Fear Conditioning Protocol

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Fear conditioning took place in a Plexiglas rodent conditioning chamber with a metal grid floor (Coulbourn Instruments, Whitehall, PA). Rats were habituated to the training chamber for 20 min 1 day before fear conditioning. On the next day, rats were allowed to acclimate to the conditioning chambers for 5 min followed by five pairings of a tone (CS; 40 s, 5 kHz, 78 dB) that was coterminated with a foot shock (US; 0.5 s, 1.3 mA). The intertrial interval (ITI) was 170–180 s. Rat groups were tested 2 h after training for short-term memory (STM) or 24 h after training for long-term memory (LTM) in a chamber with different floor, light, and odor to diminish the effect of context. Rats were given a 5 min acclimation period before the memory test and then were presented with five tones (40 s, 5 kHz, 78 dB) with ITI of 170–180 s. Behavior was recorded, and the video images were transferred to a computer equipped with FreezeFrame analysis program. The percentage of changed pixels between two adjacent 1 s images was used as a measure of activity.
Basu S., Kustanovich I, & Lamprecht R. (2016). Arp2/3 and VASP Are Essential for Fear Memory Formation in Lateral Amygdala. eNeuro, 3(6), ENEURO.0302-16.2016.
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2

Contextual Fear Conditioning in Rats

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On day 1, rats were habituated to the context A for 10 min, which consisted of a standard conditioning chamber with a metal grid floor (Coulbourn Instruments). On day 2, animals were placed in the conditioning chamber in context A for 10 min and received three signaled footshocks (0.5 mA, 1 s) preceded by a tone stimulus (80 dB, 30 s). Animals in the no shock group were placed in the conditioning chamber in context A for 10 min, heard the tone but did not receive shocks. On day 3, animals were placed in context B and received five tones (80 dB, 30 s). Context B contained a black Plexiglas floor washed with peppermint soap, different wall materials (clear plastic or metal) and different light placement. Total time spent freezing to the tone was manually scored offline for each animal by an observer blind to group assignment.
Urien L, & Bauer E.P. (2022). Sex Differences in BNST and Amygdala Activation by Contextual, Cued, and Unpredictable Threats. eNeuro, 9(1), ENEURO.0233-21.2021.
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3

Contextual and Cue Fear Memory Assay

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The FC test is used widely to assess associative learning and memory with contextual (hippocampal and amygdala-dependent) and cue (amygdala-dependent) tests43 . For training, each mouse was placed in a sound attenuating chamber with a metal grid floor (Coulbourn Instruments, White Hall, PA) and given two pairings of tone (30 sec white noise at 85 ± 2 dB) and shock (0.72 mA for 2 sec) with 2 min for habituation and 120s intersound interval. 24 hours after training, mice were observed for 5 mins in the same chamber with no sound stimulus (contextual test). Two hours after the contextual test, mice were tested for cue fear memory with 3 minutes of no sound and 3 minutes of the tone in the chamber altered to have a plastic floor covering the metal grid, changed chamber shape with plastic inserts, vanilla odor, and red light in the testing room. For each test, the mouse was recorded and percent time freezing analyzed using the Coulbourn/Actimetrics FreezeFrame3 system (Coulbourn Instruments, White Hall, PA).
Born H.A., Dao A.T., Levine A.T., Lee W.L., Mehta N.M., Mehra S., Weeber E.J, & Anderson A.E. (2017). Strain-dependence of the Angelman Syndrome phenotypes in Ube3a maternal deficiency mice. Scientific Reports, 7, 8451.
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4

Contextual Fear Conditioning in Rats

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On day 1, rats were habituated to the context for 10 min which consisted of a standard conditioning chamber with a metal grid floor (Coulbourn Instruments). On day 2, animals were placed in the conditioning chamber for 10 min and received three unsignaled footshocks (0.5 mA, 1 s). Animals in the “no shock” group were placed in conditioning chambers for 10 min on day 2 but did not receive shocks. On day 3, animals were returned to the conditioning chamber for 10 min. Total time spent freezing after the shock during conditioning (30 s) and to the context during testing (freezing time per minute over 10 min) was manually scored offline for each animal by an observer blind to group assignment.
Urien L, & Bauer E.P. (2022). Sex Differences in BNST and Amygdala Activation by Contextual, Cued, and Unpredictable Threats. eNeuro, 9(1), ENEURO.0233-21.2021.
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5

Contextual Fear Conditioning Paradigm

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All animals were habituated to the context for 10 min which consisted of a standard conditioning chamber with a metal grid floor (Coulbourn Instruments). Preexposure to the context on Day 1 and a 5 min placement in the context before the first shock on Day 2 were used to reduce any potential behavioral differences between sexes (Wiltgen et al., 2001 (link)). The next day (Day 2), animals were placed in the conditioning chamber for 10 min and received 3 unsignaled footshocks (0.5mA, 1 sec). The first footshock was delivered after 5 min in the conditioning chamber. The timing of second and third footshocks was random with at least 45 seconds between each shock. Animals in the “no-shock” group were also habituated to the chamber on Day 1. On Day 2, they were placed in conditioning chambers for 10 minutes but did not receive shocks. 24 hours later (Day 3), animals were returned to the conditioning chamber for 10 min. Total time spent freezing to the context was manually scored offline for each animal by an observer blind to group assignment. 45 minutes after the completion of behavioral testing, animals were given an overdose of sodium pentobarbital (100mg/kg) and perfused transcardially with 0.1M PBS and 4% paraformaldehyde in 0.1M PB. Animals designated as “home-cage controls” were never exposed to the conditioning chambers.
Urien L., Stein N., Ryckman A., Bell L, & Bauer E.P. (2021). Extended amygdala circuits are differentially activated by context fear conditioning in male and female rats. Neurobiology of learning and memory, 180, 107401.
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6

Contextual Fear Conditioning in Rats

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On day 1, rats were habituated to the context for 10 min which consisted of a standard conditioning chamber with a metal grid floor (Coulbourn Instruments). On day 2, animals in the “shock” group were placed in the conditioning chamber for 8 min and received 3 footshocks (0.5mA, 1 sec). Animals in the “no-shock” group were placed in conditioning chambers for the same 8 minutes on day 2 but did not receive shocks. On day 3, shock and no-shock animals were returned to the conditioning chamber for 10 min. Total time spent freezing before the shock (baseline, 30 seconds), after the shock (30 seconds, post shock) during conditioning and to the context during testing (freezing time per minute over 10 minutes) was manually scored offline for each animal by an observer blind to group assignment. Preexposure to the context on Day 1 and a 5 min placement in the context before the first shock on Day 2 were used to reduce any potential behavioral differences between sexes (Wiltgen et al., 2001 (link)). A third group of animals received no behavioral manipulation and were naïve “home cage” controls (n=5 males, n=5 females).
Urien L., Cohen S., Howard S., Yakimov A., Nordlicht R, & Bauer E.P. (2022). Aversive contexts reduce activity in the ventral subiculum-BNST pathway. Neuroscience, 496, 129-140.
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