Deoxynucleotides dntps

The different morphotypes of the isolated bacteria and fungi were characterized by macroscopically and microscopically using molecular markers. For bacteria, the 16S rDNA sequence was detected: colonies were suspended in 200 µL TE 2X with 10 mL L -1 Tween, boiled for 10 min, and then centrifuged for 2 min at 14,000 rpm. Subsequently, 5 mL of the supernatant was used to conduct PCR analysis with the primers 27F and 1492R according to the procedures by Lane (1991) and Avellaneda-Torres et al. (2015) (link). For fungi, DNA was extracted, and the internal transcribed spacer (ITS) region of the rDNA was amplified using 5 µL of the supernatant and water to a final volume Rev Bras Cienc Solo 2020;44:e0190122 of 50 µL, which also contained a buffer solution with PCR 1X, 2.0 mM MgCl 2 , 0.25 mM deoxynucleotides (dNTPs; Promega, Madison, WI), 0.2 µM ITS1, and ITS4 initiators and 2.5 U µL -1 high-efficiency Taq DNA polymerase (Invitrogen) (Płaza et al., 2004) . The DNA sequencing was performed in a 3730XL DNA Analyzer (Applied Biosystems, Macrogen, Korea) as specified by the manufacturer. The sequences were analyzed using the Basic Local Alignment Search Tool (BLAST) (Altschul et al., 1990; (link)Benson et al., 2000) (link) and Genius PRO 5.1.5 software.