Rabbit anti pparγ

Manufactured by Affinity Biosciences

Sourced in China

Rabbit anti-PPARγ is a primary antibody that specifically recognizes the peroxisome proliferator-activated receptor gamma (PPARγ) protein. PPARγ is a nuclear receptor that plays a key role in adipocyte differentiation and glucose and lipid metabolism. This antibody can be used in various immunoassay techniques to detect and quantify the expression of PPARγ in biological samples.

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PPARγ (3 citations)

3 protocols using rabbit anti pparγ

Western Blot Analysis of Protein Expression

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Tissues or cell suspensions were collected for lysis and protein extraction, and the protein concentration was determined by a BCA protein assay kit (Abcam, Shanghai, China). Samples were electrophoresed in 8–12% SDS-PAGE gels, transferred to PVDF membranes (Millipore, Hayward, CA, USA), and then incubated with primary antibodies including rabbit anti-Nrf2 (1:1000, Affinity, Changzhou, China, AF7904), mouse anti-β actin (1:10,000, Affinity, Changzhou, China, T0022), rabbit anti-Bcl-2 (1:1000, Affinity, Changzhou, China, AF6139), rabbit anti-Bax (1:1000, Affinity, Changzhou, China, AF0120), rabbit anti-Cleaved-caspase3 (1:1000, Affinity, Changzhou, China, AF7022), rabbit anti-LC3 (1:1000, Affinity, Changzhou, China, AF5402), rabbit anti-Beclin1 (1:1000, Affinity, Changzhou, China, AF5128), rabbit anti-p62 (1:1000, Affinity, Changzhou, China, AF5384), rabbit anti-NF-κB (1:1000, Affinity, Changzhou, China, AF5006), rabbit anti-PPARγ (1:1000, Affinity, Changzhou, China, AF6284), and rabbit anti-Lamin B (1:10,000, proteintech, Wuhan, China, 12987-1-AP) at 4 °C overnight and HRP-conjugated secondary antibody (proteintech, Wuhan, China, 1:5000) at room temperature for 1 h. Target bands were detected using an enhanced chemiluminescence system, and quantitative analyses were performed using the Image J software (v 1.48, National Institutes of Health, NIH, USA).

Luo J., Wang J., Zhang J., Sang A., Ye X., Cheng Z, & Li X. (2022). Nrf2 Deficiency Exacerbated CLP-Induced Pulmonary Injury and Inflammation through Autophagy- and NF-κB/PPARγ-Mediated Macrophage Polarization. Cells, 11(23), 3927.

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Carbon Tetrachloride-Induced Liver Injury

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Sch B was purchased from Selleck Chemicals (Houston, TX, USA). Carbon tetrachloride (CCl₄) was purchased from Chinasun Specialty Products Co., Ltd. (Changshu, Jiangsu, China). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-PPARγ, anti-F4/80, anti-alpha smooth muscle actin (α-SMA), anti-CD86, Lamin B1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies were obtained from Affinity Biosciences (Changzhou, Jiangsu, China). Rabbit antibodies against nuclear factor (NF)-κB and phospho-IκBα were purchased from Cell Signaling Technology (Danvers, MA, USA). Secondary antibodies used in western blotting and immunohistochemical staining were purchased from Affinity Biosciences. Secondary antibodies used in immunofluorescence staining were purchased from Abcam (Cambridge, MA, USA).

Chen Q., Bao L., Lv L., Xie F., Zhou X., Zhang H, & Zhang G. (2021). Schisandrin B regulates macrophage polarization and alleviates liver fibrosis via activation of PPARγ. Annals of Translational Medicine, 9(19), 1500.

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Western Blot Analysis of Cell Signaling

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Cells were lysed in RIPA buffer (MilliporeSigma, Cat# R0278) and heated to denature the protein. An equal amount of protein was loaded onto 4-10% polyacrylamide gel and transferred to a 0.45-µm PVDF membrane (Thermo Fisher Scientific, Inc., Cat# IPVH00010), then blocked with 5% (w/v) bovine serum albumin. The membranes were incubated with the primary antibodies at 1:1,000 dilution, respectively [rabbit anti-p53 (Affinity Biosciences Ltd., Cat# AF0879), anti-p-p53 (p53-18) (Santa Cruz Biotechnology, Inc., Cat# sc-13580), mouse anti-GAPDH (ProteinTech Group Inc., Cat# 60004-1-Ig), mouse anti-RUNX2 (Santa Cruz Biotechnology, Inc., Cat# sc-390351), mouse anti-OPN (Santa Cruz Biotechnology, Inc., Cat# sc-21742), rabbit anti-PPARγ (Affinity Biosciences Ltd., Cat# AF6284) in primary antibody diluent (Beyotime Institute of Biotechnology, Cat# P0256)]. The membranes were washed in TBST and probed with secondary antibodies, then washed in TBST for three times and scanned using the CLiNX Scan Image system (CLiNX Science Instruments).

Yao X., Li P., Deng Y., Yang Y., Luo H, & He B. (2023). Role of p53 in promoting BMP9‑induced osteogenic differentiation of mesenchymal stem cells through TGF‑β1. Experimental and Therapeutic Medicine, 25(6), 248.

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