Granzyme a cb9

Manufactured by BioLegend

Granzyme A (CB9) is a laboratory reagent produced by BioLegend. It is a serine protease involved in the induction of target cell apoptosis. The product is intended for research use only and its core function is to assist in the study of immune cell-mediated cytotoxicity.

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Lab products found in correlation

Granzyme b gb11, by BD (1 mentions) Cd244 2b4 c1.7, by Beckman Coulter (1 mentions) Nkg2c 134591, by R&D Systems (1 mentions) Cytofix cytoperm, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Perforin b d48, by BioLegend (1 mentions)

2 protocols using granzyme a cb9

Comprehensive NK Cell Phenotyping

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NK cell phenotype was analyzed with fluorochrome-conjugated antibodies against CD2 (RPA-2.10), CD11a (HI111), CD11b (ICRF44), CD27 (M-T271), CD44 (G44-26), CD57 (NK-1), CD62L (DREG-56), CD107a (H4A3), DNAM1 (DX11), KI-67 (B56), NKp44 (p44-8.1) NKp46 (9E2), NKp30 (p30-15), NKG2D (1D11), Siglec 9 (E10-286) (BD Biosciences, Franklin Lakes, NJ, USA); NKG2C (134591) (R&D Systems, Abingdon, UK); CD2 (TS1/8), CD56 (HCD56), NKp80 (5d12), CD160 (BY55), CD161 (HP-3G10), CD319 (CRACC) (162.1), and CD352 (NTBA) (NT-7) (Biolegend, San Diego, CA, USA); CD244 (2B4) (C1.7) (Beckman Coulter, Brea, CA, USA); NKG2A (REA110), Siglec 7 (REA214) (Miltenyi) and handled according to the manufacturers’ protocol. For intracellular staining of Granzyme A (CB9) (Biolegend), Granzyme B (GB11), and Perforin (dG9) (BD Biosciences), cells were washed with PBS followed by fixation and permeabilization with Cytofix/Cytoperm (BD Biosciences) and incubated for 20 min. Following fixation and permeabilization, cells were washed and stained in Permwash according to manufacturer’s protocol. All cells were acquired by a BD LSR Fortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software (FlowJo LLC, Ashland, OR, USA).

Chrobok M., Dahlberg C.I., Sayitoglu E.C., Beljanski V., Nahi H., Gilljam M., Stellan B., Sutlu T., Duru A.D, & Alici E. (2019). Functional Assessment for Clinical Use of Serum-Free Adapted NK-92 Cells. Cancers, 11(1), 69.

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Cytotoxic Lymphocyte Characterization

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Granzyme B (GB11), CD107a (H4A3)[BD Biosciences], Granzyme A (CB9), Perforin (B-D48)[Biolegend].

Phetsouphanh C., Aldridge D., Marchi E., Munier C.M., Meyerowitz J., Murray L., Van Vuuren C., Goedhals D., Fidler S., Kelleher A., Klenerman P, & Frater J. (2019). Maintenance of Functional CD57+ Cytolytic CD4+ T Cells in HIV+ Elite Controllers. Frontiers in Immunology, 10, 1844.

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