Percp cy5.5 anti cd57

Manufactured by BioLegend

PerCP-Cy5.5 anti-CD57 is a fluorescent-conjugated antibody used for flow cytometry analysis. It targets the CD57 antigen, which is expressed on a subset of T cells and natural killer cells.

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Lab products found in correlation

Anti cd3 fitc, by BioLegend (1 mentions) Anti pd 1 bv711, by BioLegend (1 mentions) Anti cd4 bv605, by BioLegend (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Pe cy7 anti slamf7, by BioLegend (1 mentions) Anti cd4 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti cd3 pe, by BD (1 mentions) Anti cd8 bv510, by BD (1 mentions)

Close spelling variants of this product

PerCP-Cy5.5 (133 citations) Anti-CD57 (4 citations) Anti-CD57- Percp/CY5.5 (2 citations) CD57 PerCP-Cy5.5 (2 citations)

2 protocols using percp cy5.5 anti cd57

Comprehensive Phenotypic Analysis of Tumor-Specific CD4 T Cells

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Tumor-specific CD4 T clones were stained systematically in PBS, 0.2% bovine serum albumin (BSA), 5 mM EDTA, and 0.2% NaN₃ with either (i) FITC anti-CD3 (BioLegend), BV605 anti-CD4 (BioLegend), ECD anti-CD8 (BD), PE-Cy7 anti-SLAMF7 (BioLegend), PerCP-Cy5.5 anti-CD57 (BioLegend), APC (allophycocyanin) anti-CD28 (BioLegend), BV711 anti–PD-1 (BioLegend), PE anti-TCRαβ (BioLegend), and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific) or (ii) FITC anti-CD3 (BioLegend), BV605 anti-CD4 (BioLegend), APC anti-TRAIL (BioLegend), PE anti-FasL (BioLegend), and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific) at 4°C for 30 min. Data were acquired on LSR II (BD Biosciences) and analyzed using FlowJo software (v.10.4.2).

Cachot A., Bilous M., Liu Y.C., Li X., Saillard M., Cenerenti M., Rockinger G.A., Wyss T., Guillaume P., Schmidt J., Genolet R., Ercolano G., Protti M.P., Reith W., Ioannidou K., de Leval L., Trapani J.A., Coukos G., Harari A., Speiser D.E., Mathis A., Gfeller D., Altug H., Romero P, & Jandus C. (2021). Tumor-specific cytolytic CD4 T cells mediate immunity against human cancer. Science Advances, 7(9), eabe3348.

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PBMC Surface Staining for Flow Cytometry

Cited 1 time

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We performed surface staining of freshly frozen PBMC from CTOT-09 study as previously published.^{25 (link)} Anti-human antibodies included: FITC-anti-CD127 (clone: A019D5; Biolegend), PE-anti-CD3 (clone: UCHT1; BD Bioscience), PerCP-Cy5.5-anti-CD57 (clone: HNK-1; Biolegend), PE-Cy7-anti-CD4 (clone: RPA-T4; eBioscience), APC-anti-CD25 (clone: 4E3; Miltenyi Biotec), APC-Cy7-anti-PD-1 (clone EH12.2H7; Biolegend), and BV510-anti-CD8 (clone: RPA-T8; BD Biosciences). Data were acquired (> 1 × 10⁶ events) on a 3-laser FACSLyric flow cytometer (BD Biosciences) and analyzed using FlowJo software.

Cravedi P., Fribourg M., Zhang W., Yi Z., Zaslavsky E., Nudelman G., Anderson L., Hartzell S., Brouard S, & Heeger P.S. (2020). Distinct peripheral blood molecular signature emerges with successful tacrolimus withdraw in kidney transplant recipients. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 20(12), 3477-3485.

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