3 4 5 dimethylthiazole 2 5 diphenyltetrazolium bromide mtt

Fetal bovine serum (FBS), penicillin-streptomycin, and the cell culture medium (RPMI 1640) were prepared from Gibco BRL, UK. The culture plates were provided by SPL (Life Sciences, Korea). Annexin V-FITC/PI kit and PI were obtained from BD Pharmingen™. Ethidium bromide, acridine orange, 3-(4,5-dimethylthiazole)-2,5-diphenyltetrazolium bromide (MTT), and Ac-DEVD-p-nitroaniline (pNA) as a chromogenic substrate of caspase-3 were prepared from Sigma-Aldrich. Caspase-9 and caspase-8 antibodies were provided by Abcam, and mouse anti-β-actin antibody was obtained from cell signaling. The secondary antirabbit and antimouse HRP-conjugate antibodies were prepared from Razi BioTech (Tehran, Iran). All other chemicals were provided by Merck (Darmstadt, Germany) and Sigma-Aldrich.

AHCC was generously provided by Amino Up Chemical Co, Ltd. (Sapporo, Japan). Fetal bovine serum (FBS) and trypsin-EDTA were purchased from GIBCO Invitrogen Co. (Carlsband, CA). The 3-(4,5-dimethylthiazole)-2,5-diphenyl tetrazolium bromide (MTT) and dimethyl sulphoxide (DMSO) were purchased from Sigma-Aldrich Co. (St. Louis, MO). Ready–to–use sandwich ELISA kits for the detection of mouse IFN-alpha/beta, IFN-gamma were purchased from eBioscience (San Diego, CA). ELISA kit for the detection of mouse IgG1 was purchased from GenWay Biotech, Inc. (San Diego, CA).