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Cf405s

Manufactured by Biotium
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The CF405S is a fluorescent dye produced by Biotium. It has an excitation maximum of 405 nm and an emission maximum of 452 nm, making it suitable for applications that require far-red fluorescence detection.

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Lab products found in correlation

Alexa 555, by Thermo Fisher Scientific (1 mentions) Coilin, by Santa Cruz Biotechnology (1 mentions) Sc 35, by Abcam (1 mentions) Ha tag, by Roche (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Lsm 780 nlo microscope, by Zeiss (1 mentions) Holo transferrin, by Merck Group (1 mentions) Dynasore, by Selleck Chemicals (1 mentions) Cf568, by Biotium (1 mentions)

Spelling variants of this product

WGA-CF405S conjugate (3 citations) Phalloidin-CF405 (2 citations) CF405s-conjugated α-Btx (2 citations)

2 protocols using cf405s

1

Multicolor immunofluorescence protocol

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Cells were transfected in 8-well glass chambers (Millipore) and fixed with 4% paraformaldehyde 24 hours later. Cells were permeabilized with 0.1% Triton-X100 and blocked with 10% donkey serum. GFP and mCherry fluoresce was detected directly. The primary antibodies used were: SC-35 (1:300; Abcam, ab11826), PML (1:50; Santa Cruz, sc-966), coilin (1:500; Santa Cruz Biotechnology, sc-32860), B23 (1:200, Santa Cruz Biotechnology, sc-56622) Myc-tag (1:500 Cell Signaling Technologies, 71D10), HA-tag (1:250; Clone 3F10, Roche, 11867423001), FK2 (1:50; Enzo Life Sciences, BML-PW8810), V5-tag (1:300, Novus Biological, NB600–379). The secondary antibodies used were Alexa 555, 647 (1:5,000; Thermo-Fisher), and CF405S (1:1000; Biotium). Samples were mounted on ProLong Gold antifade with or without DAPI and cured before imaging on a Zeiss LSM 780 NLO microscope. Images were prepared with the Fiji software.
Bouchard J.J., Otero J.H., Scott D.C., Szulc E., Martin E.W., Sabri N., Granata D., Marzahn M.R., Lindorff-Larsen K., Salvatella X., Schulman B.A, & Mittag T. (2018). Cancer mutations of the tumor suppressor SPOP disrupt the formation of active, phase-separated compartments. Molecular cell, 72(1), 19-36.e8.
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2

Holo-Transferrin Internalization Assay

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Holo-Transferrin (616397–500MG-M) was purchased from Millipore Sigma. Fluorescently conjugated Holo-Transferrin (CF®405S and CF®568) were purchased from Biotium. Dynasore (S8047) was purchased from Selleckchem. Rituximab, Cyclophosphamide, Doxorubicin, Vincristine and Prednisone were kindly provided by the Sylvester Comprehensive Cancer Center chemotherapy pharmacy.
Arumov A., Liyanage P.Y., Trabolsi A., Roberts E.R., Li L., Ferreira B.C., Gao Z., Ban Y., Newsam A.D., Taggart M.W., Vega F., Bilbao D., Leblanc R.M, & Schatz J.H. (2020). Optimized doxorubicin chemotherapy for diffuse Large B-Cell lymphoma exploits nanocarrier delivery to transferrin receptors. Cancer research, 81(3), 763-775.
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