Cleaved caspase 3 elisa kit

Manufactured by Cell Signaling Technology

Sourced in United States

The Cleaved caspase-3 ELISA kit is a quantitative immunoassay designed for the measurement of cleaved caspase-3 in cell lysates and other biological samples.

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Lab products found in correlation

One step rt pcr kit, by Thermo Fisher Scientific (1 mentions) P perk, by Cell Signaling Technology (1 mentions) Carnosol, by Cayman Chemical (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Transit siquest transfection reagent, by Mirus Bio (1 mentions) Rnase free dnase set, by Qiagen (1 mentions) Deadend fluorometric tunel system, by Promega (1 mentions) Bacterial sphingomyelinase, by Merck Group (1 mentions) Fluo 4 nw calcium assay kit, by Thermo Fisher Scientific (1 mentions) Silica gel 60 thin layer chromatography plate, by Merck Group (1 mentions) 2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions) Fumonisin b1, by Merck Group (1 mentions) Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions) Trolox, by Bio-Techne (1 mentions) C6 ceramide, by Merck Group (1 mentions) Bradford reagent, by Bio-Rad (1 mentions)

3 protocols using cleaved caspase 3 elisa kit

Carnosol Modulation of Nrf2 Signaling

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Carnosol was obtained from Cayman Chemical Co. (Ann Arbor, MI. Lot: 127606–37, Purity ≥ 99%). All antibodies for western blot analysis and cleaved caspase-3 ELISA kit were obtained from Cell Signaling Technology (Danvers, MA). DeadEnd™ Fluorometric TUNEL System was obtained from Promega (Madison, WI). BCA protein assay kit, chemiluminescent, NE-PER nuclear and cytoplasmic extraction reagents were obtained from Pierce (Rockford, IL). Nrf2 antibody (sc-722) for immunofluorescence was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Fluorescein isothiocyanate (FITC)-conjugated goat-anti-rabbit antibody was obtained from Pierce (Rockford, IL). Antibodies purchased from Cell Signal Technology are listed with the catalog number in parentheses: Nrf2 (12721P), Erk (9911), Sestrin-2 (8487S), GPX1 (3206S), GSTP1 (3369S), SOD1 (2770S), PERK (5638P), p-PERK (3179S), and HO-1 (5853S). One-Step RT-PCR kit was obtained from Life Technologies (Grand Island, NY). RNeasy mini kit and RNase-Free DNase set for RNA extraction were obtained from QIAGEN (Santa Clarita, CA). TransIT-siQUEST transfection reagent was obtained from Mirus Bio LLC. Nrf2 siRNA(h) and Control siRNA-A were obtained from Santa Cruz Bio, Inc.

Yan M., Vemu B., Veenstra J., Petiwala S.M, & Johnson J.J. (2018). Carnosol, a dietary diterpene from rosemary (Rosmarinus officinalis) activates Nrf2 leading to sestrin 2 induction in colon cells. Integrative molecular medicine, 5(4), 10.15761/IMM.1000335.

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Quantifying Cleaved Caspase-3 in Cells and Tissue

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A cleaved caspase-3 ELISA kit (Cell Signaling Technologies, USA) was used to measure endogenous levels of cleaved caspase-3 protein in vivo and in vitro. Briefly, astrocytes or brain tissue lysis buffer were centrifuged at 14,000 rpm for 10 min, and the astrocyte supernatant was transferred to a new tube and analyzed according to the manufacturer’s instructions. Absorbance was assessed at 450 nm within 30 min using an ELISA plate reader. The results were described as activity relative to that in the control group.

Zhuang F., Shi X., Qiao S., Liu B., Wang Z., Huo H., Liang F., Shen L., Zhu L., He B, & Wang H. (2023). Allicin promotes functional recovery in ischemic stroke via glutathione peroxidase-1 activation of Src-Akt-Erk. Cell Death Discovery, 9, 335.

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Thrombin-Induced Lipid Signaling Pathway

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α-Thrombin (human plasma, ≥2,800 NIH units/mg protein) was purchased from Calbiochem (La Jolla, CA, USA) and Sigma-Aldrich (St. Louis, MO, USA). [9,10-³H(N)]palmitic acid, [5,6,7,8,9,11,12,14,15-³H(N)]arachidonic acid was purchased from Amersham Biosciences (Buckinghamshire, England, UK). MTT (Thiazolyl Blue Tetrazolium Bromide), C₆-ceramide, bacterial sphingomyelinase, and 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich. BAPTA-AM (1,2-bis-(o-Aminophenoxy)-ethane-N,N,N’,N’-tetraacetic acid, tetraacetoxymethyl ester) was purchased from BIOMOL Research Labs., Inc (Ann Arbor, MI, USA). Silica gel 60 thin-layer chromatography (TLC) plates were purchased from Merck (Darmstadt, Germany). For the protein assay, the Bradford reagent was purchased from Bio-Rad (Hercules, CA, USA). Plasticware for tissue culture was purchased from NUNC (Waltham, MA, USA). The cleaved caspase-3 ELISA kit was purchased from Cell Signaling Technology (Danvers, MA, USA). AA and Fumonisin B₁ were purchased from Sigma-Aldrich. Trolox was obtained from Tocris (Minneapolis, MN, USA) and the Fluo-4 NW Calcium Assay Kit was purchased from Invitrogen (Carlsbad, CA, USA).

Park M.J., Won J.H, & Kim D.K. (2022). Thrombin Induced Apoptosis through Calcium-Mediated Activation of Cytosolic Phospholipase A2 in Intestinal Myofibroblasts. Biomolecules & Therapeutics, 31(1), 59-67.

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