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3 protocols using n octanol

1

Magnolol and Honokiol Extraction Protocol

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Magnolol and honokiol (98% purity) were acquired from New Natural Biotechnology (Shanghai, China). Phosphotungstic acid solution, sodium phosphate monobasic and potassium phosphate monobasic were purchased from Sigma-Aldrich (Madrid, Spain). N-octanol, acetonitrile, ethyl alcohol, and potassium chloride were obtained from VWR chemicals (Barcelona, Spain). Hydrochloric acid 37% and sodium hydroxide were purchased from Scharlab (Barcelona, Spain). Hydrogen peroxide 30% and boric acid were obtained from Merck (Barcelona, Spain). Phospholipon 90 G was provided by Lipoid GmbH (Ludwigshafen, Germany).
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2

Cationic and Nonionic Surfactant Interactions

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Alkyltrimethylammonium
bromides (CnTAB, where n is the number of carbon atoms in the alkyl chain), i.e., octyltrimethylammonium
bromide (n = 8), dodecyltrimethylammonium bromide
(n = 12), cetyltrimethylammonium bromide (n = 16), and octadecyltrimethylammonium bromide (n = 18) of highest purity (≥98%, Sigma-Aldrich),
were used as cationic surfactants, while n-octanol
(≥98%, VWR) was used as a nonionic surfactant.
Particles
of high-purity quartz (>98% SiO2) of size 50–100
μm were used in floatability tests.
Milli-Q water (18.2
MΩ·cm) was used for cleaning all
parts of the experimental setup and preparation of pure solutions
of cationic surfactants and their mixtures with a nonionic surface-active
substance used in the experiments.
The experiment was designed
in such a way that the concentration
of CnTAB, as the solution main component,
was changed in a quite broad range, while the concentration of n-octanol (nonionic additive) was kept constant and equal
to 5 × 10–4 M. This particular concentration
was chosen as the lowest, having a significant and easily measurable
synergistic influence on the measured parameters.12 (link)
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3

HPLC Analysis of Pharmacological Compounds

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AZA, 6-MP, 6-TG, FA, dimethylsulfoxide and phosphate buffered saline (PBS) tablets, all HPLC grade, were obtained from Sigma Aldrich (Germany). Acetonitrile, HPLC grade, was obtained from J. T. Baker (Deventer, The Netherlands). Formic acid, HPLC grade, was purchased from Merck (Germany). n-octanol, purified, was obtained from VWR (France). Ultrapure water was prepared with a Mili-Q water purification system (Millipore, USA).
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