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3 protocols using 3 hydroxycarbofuran

1

Carbofuran and Metabolite Quantification

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Carbofuran (purity, 98%) and 3-hydroxyCarbofuran (purity, 99%) were purchased from Sigma Aldrich (Milwaukee, WI, USA). All solvents used for QuEChERS extraction and LC–MS operation were of LC–MS grade and purchased from Fisher Scientific (Pittsburgh, PA, USA). All other chemicals used were of reagent grade unless otherwise noted.
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2

Pesticide Residue Analysis Protocol

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A cocktail mix containing 8 OP residues (dichlorvos), MOCAP (ethoprophos), disulfoton, methyl parathion, Ronnel, Dursban (Chlorpyrifos), guthion (azinphos methyl), and Tokuthion and a standard mix of 10 CB residues (aldicarb sulfoxide), aldicarb sulfone, oxamyl, methomyl, 3-hydroxycarbofuran, aldicarb, propoxur, carbofuran, Sevin (carbaryl), and methiocarb were purchased from Sigma-Aldrich, USA. Other chemicals including n-hexane, ethyl acetate, acetone, sodium chloride, and anhydrous sodium sulphate were brought from Sigma Aldrich, Germany.
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3

Quantitative Analysis of Carbofuran and Metabolites

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Carbofuran, 3-hydroxyCarbofuran, and diazepam-d5 (internal standard), all purity >98% (Fig. 1), were purchased from Sigma-Aldrich Co. LLC (St. Louis, MO USA). Acetonitrile and methanol (HPLC grade) were purchased from Merck (Darmstadt, Germany). Ultrapure water was prepared by a Millipore Milli-Q purification system (Bedford, MA, USA).
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