S prg filler

Manufactured by Shofu

Sourced in Japan

The S-PRG filler is a dental restorative material developed by Shofu. It is a light-cured, pre-reacted glass ionomer-based composite that provides fluoride release and recharge.

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Lab products found in correlation

Icps 8000, by Shimadzu (1 mentions)

5 protocols using s prg filler

Characterization of Ion Release from S-PRG Filler

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S-PRG eluates were formulated by mixing DW with S-PRG filler (Shofu Inc., Kyoto, Japan) in a 1:1 ratio (1 L: 1,000 g), followed by stirring at 23 °C for 24 h. And the mixture was centrifuged to precipitate the S-PRG filler. The supernatant solution was then filtered using a Chromato Disk (25A Hydrophilic Type, Diameter: 25 mm, pore size: 0.2 µm; GL Sciences Inc., Tokyo, Japan) to obtain the various solution concentrations (25%, 50%, and 100%)^{27 (link)}. Furthermore, the elemental analyses of the BO₃^3–, Na⁺, Sr²⁺, Al³⁺, SiO₃^2–, and F^– ions released from the S-PRG fillers were performed using inductively coupled plasma atomic emission spectroscopy (ICPS-8000, Shimadzu Corp., Kyoto, Japan) and fluoride ion electrodes (Model 9609BNWP, Orion Research, Boston, MA, USA) (Table 2).Table 2

Concentrations of ions released from S-PRG filler.

	BO₃³⁻	Na⁺	Sr²⁺	F⁻	Al³⁺	SiO₃²⁻
25% S-PRG	348.3	97.9	34.2	28.6	2.4	2.7
50% S-PRG	696.6	195.8	68.3	57.3	4.9	5.4
100% S-PRG	1,393.2	391.6	136.7	114.5	9.7	10.8

Concentration values are in parts per million.

Kim H.J., Cho M.Y., Lee E.S., Jung H.I, & Kim B.I. (2020). Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm. Scientific Reports, 10, 14425.

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Antimicrobial Effects of S-PRG Eluate

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Strains tested this study were S. mutans ATCC25175 and OMZ175, S. oralis ATCC6249 and ATCC10557, S. gordonii Challis and ST202, A. naeslundii ATCC12104 and B74, P. gingivalis ATCC33277 and FDC381, F. nucleatum ATCC25586 and JCM11023, and A. actinomycetemcomitans Y4 and ATCC33348.
S-PRG filler, produced according to a previously published method [21 (link)] was obtained from SHOFU Inc. (Kyoto, Japan). Briefly, S-PRG filler was mixed with an equal amount of distilled water under constant stirring for 24 h. Subsequently, we centrifuged and used filtered supernatant. Recovered filtrate (S-PRG eluate; SPE) served as the test sample used throughout this study.

Kono Y., Tamura M., Cueno M.E., Tonogi M, & Imai K. (2021). S-PRG Filler Eluate Induces Oxidative Stress in Oral Microorganism: Suppression of Growth and Pathogenicity, and Possible Clinical Application. Antibiotics, 10(7), 816.

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Preparation of S-PRG Eluate

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S‐PRG eluate was prepared according to the method described by Fujimoto et al. (Fujimoto et al., 2010). Briefly, S‐PRG filler (SHOFU Inc., Kyoto, Japan) was mixed with an equal amount of distilled water and shaken gently at room temperature for 24 hr. The filler material was removed by filtration, and the ion solution was centrifuged to remove any residual insoluble material. The clear supernatant was collected and used as the S‐PRG eluate. The ion concentration of S‐PRG was as follows: Al, 12.1 ppm; B, 2001.1 ppm; Na, 544.5 ppm; Si, 14.0 ppm; Sr, 103.5 ppm; and F, 110.5 ppm. We used this eluate throughout the manuscript.

Iwamatsu‐Kobayashi Y., Abe S., Fujieda Y., Orimoto A., Kanehira M., Handa K., Venkataiah V.S., Zou W., Ishikawa M, & Saito M. (2017). Metal ions from S‐PRG filler have the potential to prevent periodontal disease. Clinical and Experimental Dental Research, 3(4), 126-133.

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Antifungal Efficacy of S-PRG Filler Eluate

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The S-PRG filler with a particle size of 1 µm (Shofu, Kyoto, Japan) was immersed in distilled water at a concentration of 1,000 g/L for 24 h, and then, the liquid was filtered, collected, and diluted. According to Tamura et al., the maximum dilution factors that have little effect on growth are 1:16 and 1:32 16) . Therefore, these concentrations were tested in this study. Distilled water was used as a control.
LIP expression analysis 1. Treatment of C. albicans with the S-PRG filler eluate Culture media were prepared by adding S-PRG eluate at dilution factors of 1:16 or 1:32 to 2× yeast extract peptone dextrose broth. The final dilution ratios of the S-PRG filler elutes were 1:32 and 1:64. Subsequently, the prepared medium of the experimental treatment and control (distilled water) group was inoculated with the prepared fungal suspension and incubated at 37°C for 24 h.

Tonprasong W., Inokoshi M., Tamura M., Hatano K, & Minakuchi S. (2023). Impact of surface pre-reacted glass ionomer filler eluate on lipase gene expression in Candida albicans: An in vitro study. Dental materials journal, 42(1).

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Fabrication of S-PRG Filler-Containing EVA Materials

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Experimental EVA materials containing S-PRG filler S-PRG filler (Shofu, Kyoto, Japan) was prepared as described previously 15, 27) . S-PRG filler was added to EVA copolymer (EVAFLEX ® EV360, Du Pon-Mitsui Polychemicals, Tokyo, Japan) at 5, 10, 20, or 40 wt%, and mixed for 10 min at 110°C. The mixture was processed into sheets (thickness: 4 mm) using a mill roll, then heat pressed for 8 min at 160°C. Disc-shaped test specimens (diameter: 8 mm, thickness: 4 mm) were prepared from the S-PRG filler-containing EVA sheets. Control specimens of the same size were also prepared from 4 mm-thick EVA sheets without S-PRG filler.

Nagai K., Domon H., Oda M., Shirai T., Ohsumi T., Terao Y, & Arai Y. (2017). Antimicrobial activity of ethylene-vinyl acetate containing bioactive filler against oral bacteria. Dental materials journal, 36(6).

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