T12 transmission electron microscope

Manufactured by Thermo Fisher Scientific

Sourced in United States

The T12 transmission electron microscope is a laboratory instrument designed for the high-resolution imaging of thin samples. It utilizes a focused beam of electrons to illuminate the specimen and create a magnified image, providing researchers with detailed information about the internal structure and composition of materials at the nanoscale level. The core function of the T12 is to enable the observation and analysis of a wide range of samples, from biological specimens to advanced materials.

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Lab products found in correlation

Ls c72966, by LSBio (2 mentions) Goat anti rabbit igg conjugated to 20 nm gold particles, by Electron Microscopy Sciences (2 mentions) Epon resin, by Merck Group (1 mentions) Phosphotungstic acid, by Merck Group (1 mentions) Uv 2600, by Shimadzu (1 mentions) Tecnai g2 f30, by Thermo Fisher Scientific (1 mentions) 4 k ccd camera, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Transmission electron microscope (80 citations) Tecnai T12 transmission electron microscope (44 citations) T12 electron microscope (22 citations) T12 microscope (19 citations) Tecnai Spirit (T12) transmission electron microscope (5 citations) Tecnai T12 Transmission Electron Microscope (TEM (3 citations) T12 Bio-TWIN transmission electron microscope (3 citations) Technai T12 transmission electron microscope (2 citations) Tecnai T12 120kV Transmission Electron Microscope (2 citations) Tecnai T12 G2 TWIN transmission electron microscope (2 citations)

15 protocols using t12 transmission electron microscope

Extracellular Vesicle Visualization via TEM

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Purified SEVs reconstituted in 20 mM HEPES buffer were added on glow-discharged formvar carbon film-coated grids in 10 μL volume for 1 min at room temperature. Subsequently, grids were stained with 2% uranyl acetate for 30 s and were allowed to air dry. Grids were imaged using a Philips/FEI T-12 transmission electron microscope at 21000x magnification.

Patel M.R, & Weaver A.M. (2021). Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-β signaling. Cell reports, 34(10), 108829.

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Extracellular Vesicle Visualization via TEM

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Patel M.R, & Weaver A.M. (2021). Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-β signaling. Cell reports, 34(10), 108829.

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Tissue Fixation and Electron Microscopy

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Human tissue samples from cases and controls were harvested in the operating theater and immediately fixed with chilled buffer (50 mM sodium cacodylate [pH 7.4]) containing 2.5% glutaraldehyde and 2.0% paraformaldehyde and placed in 4°C overnight. The samples were then prepared as previsouly described^{9 (link)}. Samples analyzed with an FEI T-12 transmission electron microscope equipped with a side-mounted digital camera.

Gelbard A., Katsantonis N.G., Mizuta M., Newcomb D., Rotsinger J., Rousseau B., Daniero J.J., Edell E.S., Ekbom D.C., Kasperbauer J.L., Hillel A.T., Yang L., Garrett C.G., Netterville J.L., Wootten C.T., Francis D.O., Stratton C., Jenkins K., McGreggor T.L., Gaddy J.A., Blackwell T.S, & Drake W.P. (2016). Idiopathic Subglottic Stenosis is Associated with Activation of the Inflammatory IL-17A/IL23 axis: North American Airway Collaborative (NoAAC) TS-03 study. The Laryngoscope, 126(11), E356-E361.

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Imaging Mycobacterial Infections in Human Tracheal Tissue

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Human tracheal mucosal biopsies were obtained in the operating theater and immediately fixed with chilled buffer (50 mM sodium cacodylate [pH 7.4]) containing 2.5% glutaraldehyde and 2.0% paraformaldehyde and placed in 4°C overnight. The samples were then prepared as previsouly described^{27 (link)}. Briefly, samples were blocked with 0.1% coldwater fish skin gelatin in 50 mM sodium cacodylate buffer and stained with rabbit polyclonal anti-Mtb antibodies (LS-C72966, LSBio, Inc.), followed by goat anti-rabbit IgG conjugated to 20 nm gold particles (Electron Microscopy Sciences). Samples were washed three times with phosphate buffered saline containing 0.1% Tween 20 (PBS-T) and analyzed with an FEI T-12 transmission electron microscope equipped with a side-mounted digital camera. A total of 30-35 individual cells in each group were imaged to analyze subcellular architecture and presence of bacteria.

Gelbard A., Katsantonis N., Mizuta M., Newcomb D., Rotsinger J., Rousseau B., Daniero J.J., Edell E.S., Ekbom D.C., Kasperbauer J.L., Hillel A.T., Yang L., Garrett C.G., Netterville J.L., Wootten C.T., Francis D.O., Stratton C., Jenkins K., McGregor T.L., Gaddy J.A., Blackwell T.S, & Drake W.P. (2016). Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species. The Laryngoscope, 127(1), 179-185.

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TEM Imaging of Cu-Treated E. coli Cells

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E. coli cells that had been treated with 0.1 mg/mL Cu species for 24 hours were washed 3 times with phosphate-buffered saline (PBS) before being fixed with 2% glutaraldehyde in 0.1 M PBS. Cells were washed with PBS 3 times before treating with osmium tetroxide (OsO₄) in PBS for 1 hr. After rinsing 3 times with PBS, the cell pellet was dehydrated in a graded series of ethanol (30%, 50%, 70%, 80%, 90%, 95%, and 100% ethanol for two hours each). Propylene oxide was used to remove any residual ethanol before the pellet was embedded in Epon resin (Sigma Aldrich). A Reichert-Jung Ultracut E ultramicrotome was used to cut 100 nm thick sections which were placed onto 1GG200 gold grids (Ted Pell Inc.). Sections were stained with uranyl acetate and Reynolds lead citrate, and examined on an FEI T12 transmission electron microscope operated at 80 kV in the Electron Imaging Center for Nanomachines (EICN) at UCLA.

Kaweeteerawat C., Chang C.H., Roy K.R., Liu R., Li R., Toso D., Fischer H., Ivask A., Ji Z., Zink J.I., Zhou Z.H., Chanfreau G.F., Telesca D., Cohen Y., Ann Holden P., Nel A.E, & Godwin H.A. (2015). Cu Nanoparticles Have Different Impacts in E. coli and L. brevis than Their Micron-Sized and Ionic Analogs. ACS nano, 9(7), 7215-7225.

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Negative Staining TEM Imaging Protocol

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Samples were first fixed with 2.5% gluteraldehyde in 0.1M sodium cacodylate buffer at RT for 30 min. Samples were then deposited on formvar carbon-coated grids (Electron Microscopy Sciences, Hatfield, PA) for 1 min followed by negative staining with 2% uranyl acetate for 30s at RT. Imaging was performed on a Philips/FEI T-12 Transmission Electron Microscope. Micrographs were captured with a 2k × 2k CCD camera (Advanced Microscopy Techniques, Woburn, MA).TEM was performed in part through the use of the Vanderbilt Cell Imaging Shared Resource (supported by NIH grants CA68485, DK20593, DK58404, DK59637 and EY08126).

Jeppesen D.K., Fenix A.M., Franklin J.L., Higginbotham J.N., Zhang Q., Zimmerman L.J., Liebler D.C., Ping J., Liu Q., Evans R., Fissell W.H., Patton J.G., Rome L.H., Burnette D.T, & Coffey R.J. (2019). Reassessment of Exosome Composition. Cell, 177(2), 428-445.e18.

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Visualizing Bacterial Infection via SEM and TEM

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SEM and TEM were performed to confirm localization and view cell–cell interactions^{70 (link)}. For TEM, VK2 E6/E7 cells were grown in six-well tissue culture plates and infected with strain UTI89 at the indicated MOI as described for the adherence and invasion assay. At the indicated time point cells were washed, and fixed in 2.5% glutaraldehyde in 100 mM sodium cacodylate for 30 min at room temperature. Specimens were placed on glow-discharged formvar/carbon-coated copper grids and stained with 1% uranyl acetate for 90 s. A Philips/FEI T-12 transmission electron microscope was used for sample analysis. For SEM, VK2 E6/E7 cells were grown on glass cover slips in 12-well plates, infected with the bacteria for 2 h and fixed with 2.5% glutaraldehyde in 100 mM sodium cacodylate for 30 min at room temperature. Samples were critical point dried and placed onto aluminum stubs prior to sputter coating with gold-palladium. Images were collected for each sample and representative images were taken with a FEI Quanta 250 field-emission gun scanning electron microscope (Field Electron and Ion Company, Hilllsboro, OR). Images were taken from a minimum of three biological replicates.

Brannon J.R., Dunigan T.L., Beebout C.J., Ross T., Wiebe M.A., Reynolds W.S, & Hadjifrangiskou M. (2020). Invasion of vaginal epithelial cells by uropathogenic Escherichia coli. Nature Communications, 11, 2803.

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Morphological Analysis of APCE94 Strains

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Morphological changes of the APCE94, APCE94ΔeivC, and APCE94CΔeivC strains were determined by transmission electron microscopy (TEM). Briefly, each strain was grown on LB agar plates and suspended and washed with phosphate-buffered saline (PBS). Then, the bacterial pellets were negatively stained using 2% phosphotungstic acid (Sigma). Finally, the stained bacteria were deposited on a carbon-coated grid, followed by observation under a FEI T12 transmission electron microscope (FEI, Ltd, Hillsboro, OR, USA).

Wang S., Liu X., Xu X., Yang D., Wang D., Han X., Shi Y., Tian M., Ding C., Peng D, & Yu S. (2016). Escherichia coli Type III Secretion System 2 ATPase EivC Is Involved in the Motility and Virulence of Avian Pathogenic Escherichia coli. Frontiers in Microbiology, 7, 1387.

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Phage Morphology Analysis by TEM

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Phage morphology was analyzed through transmission electron microscopy (TEM). The filtered high-titer phage particles were dropped on a copper grid and negatively stained with 2% phosphotungstic acid. Excess liquid was blotted off and then the grids were observed under a FEI T12 transmission electron microscope (FEI, Ltd, Hillsboro, OR, USA).

Yao L., Bao Y., Hu J., Zhang B., Wang Z., Wang X., Guo W., Wang D., Qi J., Tian M., Bao Y., Li H, & Wang S. (2023). A lytic phage to control multidrug-resistant avian pathogenic Escherichia coli (APEC) infection. Frontiers in Cellular and Infection Microbiology, 13, 1253815.

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Negative Staining TEM Imaging of Exosomes

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For negative staining microscopy, 10 μL of exosomes (1 μg/μL) were layered and absorbed on a formvarcoated 400 mesh copper grid and stained with 1% PTA. Sample was imaged using a FEI tecnai T12 transmission electron microscope (FEI, Hillsboro, OR) equipped with a thermionic Lab6 filament and operated at an acceleration of 80 kV. Images were taken with a pixel size of 0.46 nm and a direct magnification of 21 000× using an AMT CCD camera.

Clark D.J., Fondrie W.E., Liao Z., Hanson P.I., Fulton A., Mao L, & Yang A.J. (2015). Redefining the Breast Cancer Exosome Proteome by Tandem Mass Tag Quantitative Proteomics and Multivariate Cluster Analysis. Analytical chemistry, 87(20), 10462-10469.

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