Anti nt 3 sc 547

We used the following antibodies: anti-GFP (A11122, Life technologies), anti-GAPDH (sc-25778, Santa Cruz), anti-Histone (07–449, Millipore), anti-Flag (F3165, Sigma), anti-Actin (MAB1501R, Chemicon), anti-Hey1 (anti-HRT1, sc-16424, Santa Cruz), anti-p53 (SAPU, [53 (link)]), anti-Ptc (sc-6149, Santa Cruz), anti-TrkC (AF1404, R&D), anti-MDM2 (VMA00406, BioRad), anti-COBRA1 (F7E4, GeneTex), anti-BAX (sc-526, Tebu Bio), anti-BAK (G-23, Santa Cruz), anti-NT-3 (sc-547, Santa Cruz), and anticleaved PARP (9541T, Cell Signaling). The following antibodies were used for proximity ligation assays (DuoLink): anti-GFP (TP401, Biolabs), anti-Flag (F3165, Sigma), anti-Hey1 (anti-HRT1, sc-16424, Santa Cruz), anti-p53 (sc-126, Santa Cruz), and anti-importins (I1784, Sigma Aldrich).

Coon’s modified Ham’s F-12 medium and human insulin were purchased from Sigma Chemical Co (St Louis, MO, USA). Penicillin/streptomycin solution, neurobasal medium, and B27 were purchased from Gibco (Grand Island, NY, USA), and bFGF, recombinant rat IL-1β, and recombinant Wnt5a were purchased from R&D Systems (Minneapolis, MN, USA). The following antibodies were purchased: anti NT3 (#SC-547) and anti Ngn1 (#SC-19231) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti p-JNK (#9251S), anti JNK (#9252S), anti p-NF-κB (#3031S) and anti NF-κB (#6956S) from Cell Signaling Technology (Beverly, MA, USA); anti Wnt5a (#MABD136) from Merck (Darmstadt, Germany); anti β-tubulin type III (TUJ1) (#801202) from BioLegend (San Diego, CA, USA); anti calnexin (#ADI-SPA-860-F) from Enzo Life Sciences (Farmingdale, NY, USA). Fluorescein-(DTAF)-conjugated streptavidin (#016–010-084) was purchased from Jackson Immuno Research (Westgrove, PA, USA). Y27632 and SP600125 were purchased from Abcam (Cambridge, UK).