Mirvana paris protocol

Manufactured by Thermo Fisher Scientific

The MiRVana PARIS protocol is a laboratory technique for the isolation and purification of total RNA, including small RNAs such as microRNAs (miRNAs), from various biological samples. The protocol utilizes a combination of guanidinium-based lysis and silica-based column purification to efficiently extract and purify RNA molecules of all sizes.

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Lab products found in correlation

Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Mirvana kit, by Thermo Fisher Scientific (1 mentions) Flow cell, by Illumina (1 mentions) Truseq small rna kit, by Illumina (1 mentions) Agilent high sensitivity dna kit, by Agilent Technologies (1 mentions) R1016, by Zymo Research (1 mentions)

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MirVana PARIS

2 protocols using mirvana paris protocol

Extraction of miRNA from Liver and Serum

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For liver samples, the miRVana kit (AM1560; Life Technologies) was used according to the manufacturer's protocol with a slight modification for two phenol chloroform extractions (Burgos et al., 2013). RNA concentrations were measured using a NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA). Serum samples (1–2 ml) were isolated using the same modified miRVana PARIS protocol (AM1556; Life Technologies) with sequential phenol–chloroform extractions 13.

Van Keuren‐Jensen K.R., Malenica I., Courtright A.L., Ghaffari L.T., Starr A.P., Metpally R.P., Beecroft T.A., Carlson E.W., Kiefer J.A., Pockros P.J, & Rakela J. (2015). microRNA changes in liver tissue associated with fibrosis progression in patients with hepatitis C. Liver International, 36(3), 334-343.

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Plasma miRNA Extraction and Sequencing

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The samples employed were previously published (Shah et al., 2017 (link)). All subjects provided written informed consent under approved Institutional Review Board protocols at the Beth Israel Deaconess Medical Center and Massachusetts General Hospital. Plasma samples (1 ml) were isolated using a modified mirVana PARIS protocol (AM1556, Life Technologies) with sequential phenol-chloroform extractions (Burgos et al., 2013 (link)). RNA was cleaned and concentrated (R1016, Zymo Research, Irvine, CA). Sample preparation was done using the Illumina small RNA TruSeq kit (RS-200-0048, Illumina). The kit reagents were halved at all steps with 16 rounds of PCR amplification. Libraires were quantified with the Agilent High Sensitivity DNA Kit (5067-4626, Agilent) and sequenced on an Illumina flowcell.

Alsop E., Meechoovet B., Kitchen R., Sweeney T., Beach T.G., Serrano G.E., Hutchins E., Ghiran I., Reiman R., Syring M., Hsieh M., Courtright-Lim A., Valkov N., Whitsett T.G., Rakela J., Pockros P., Rozowsky J., Gallego J., Huentelman M.J., Shah R., Nakaji P., Kalani M.Y., Laurent L., Das S, & Van Keuren-Jensen K. (2022). A Novel Tissue Atlas and Online Tool for the Interrogation of Small RNA Expression in Human Tissues and Biofluids. Frontiers in Cell and Developmental Biology, 10, 804164.

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