Diosmetin

Neochlorogenic acid (3-CQA) (4), chlorogenic acid (5-CQA) (6), apigenin (30), luteolin (31), quercetin (35), isoquercitrin (49), hyperoside (50), luteolin-7-O-rutinoside (54), and rutin (55) were obtained from Extrasynthese (Genay, France). 3,4-O-diCQA (12), 3,4-O-diCQA (13), diosmetin (33), rhamnetin (37), isorhamnetin (38), luteolin-7-O-glucuronide (47), isorhamnetin-7-O-glucoside (51), and isorhamnetin-3-O-rutinoside (57) were purchased from PhytoLab (Vestenbergsgreuth, Germany).
The UHPLC-ESI/HRMS analyses were carried out on a Q Exactive Plus heated electrospray ionization (HESI-II) – high resolution mass spectrometer (HRMS) (ThermoFisher Scientific, Inc., Bremen, Germany) equipped with an ultra-high-performance liquid chromatography (UHPLC) system Dionex Ultimate 3000RSLC (ThermoFisher Scientific, Inc.) [60 (link)].

Formic acid (>98% purity), acetonitrile (LC-MS grade), methanol, rutin, quercetin, apigenin and kaempferol were purchased from Merck (Darmstadt, Germany). apigenin 7-O-glucoside, quercetin 3-O-glucoside, kaempferol 3-O-rutinoside and luteolin were purchased from Extrasynthese (Genay Cedex, France). Cynarin, chlorogenic acid, 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, luteolin 7-O-glucuronide, apigenin, 7-O-glucoside, luteolin 7-O-glucoside and diosmetin were purchased from Phytolab (Vestenbergsgreuth, Germany). Bond Elut Jr 500 mg SPE-C18 cartridge was from Agilent (Santa Clara, California, USA). A Milli-Q purification system (Millipore, Bedford, MA, USA) was used to obtain the deionised water (18.2 MΩ cm). All chemicals used for biological assays were obtained as pure commercial products from Sigma Chemical Co (St. Louis, USA) and used without further purification. Spectrophotometric determinations were obtained with an Ultrospec 2100 spectrophotometer (Biochrom Ltd, Cambridge, England) using a 1 cm length path cell.