Isopropyl alcohol

The following materials and reagents were used: Nafion^® 212 membrane (equivalent weight 1100, The Chemours Company, Wilmington, DE, USA), N-methyl-2-pyrrolidone (Merck, Darmstadt, Germany), N,N-dimethylformamide (special purity, Chimmed, Moscow, Russia), ethylene glycol (special purity, Chimmed, Moscow, Russia), isopropyl alcohol (special purity, Chimmed, Moscow, Russia), lithium hydroxide (LiOH, ≥99.0%, Sigma-Aldrich, St. Louis, MO, USA), hydrochloric acid (special purity, 35–38%, Chimmed, Moscow, Russia), potassium chloride (reagent grade, Chimmed, Moscow, Russia), potassium hydroxide (reagent grade, Chimmed, Moscow, Russia); deionized water (resistance 18.2 MOhm).

Ammonium cerium(IV) nitrate (99.9%, Lanhit (Moscow, Russia)), isopropyl alcohol (99.9%, Chimmed (Moscow, Russia)), hydrogen peroxide (99.9%, Chimmed), luminol (Sigma-Aldrich, St. Louis, MO, USA, 123072), potassium dihydrogen phosphate (Sigma-Aldrich, P0662), potassium hydrogen phosphate (Sigma-Aldrich, P5655), tris-hydrochloride (Merck, Lowe, NJ, USA, 10812846001), and Milli-Ω Water (18.2 MΩ/cm).

For preparing RC-containing MPs (RC MPs) as a source of PS was used a concentrate for the infusion solution “Radachlorin^Ò” manufactured by RADA-PHARMA (Moscow, Russia), comprising 3.5 mg/mL of a mixture of sodium salts of chlorin e6, chlorin p6 and purpurin 5. Poly(lactic-co-glycolic acid) 65:35 copolymer (PLGA) RESOMER RG 653 H was purchased from Evonik Industries AG (Essen, Germany); span 80, Polysorbate 20, light mineral (paraffin) oil, 1,3-diphenylisobenzofuran (DPBF) were from Sigma (Saint Louis, MO, USA); polyvinyl alcohol 18-88 was from Merck (Darmstadt, Germany); methylcellulose A4M was from Ashland (Covington, KY, USA); DMEM12 medium, penicillin, streptomycin, L-glutamine, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were from PanEco (Moscow, Russia); fetal bovine serum was from Hyclone (Chicago, IL, USA). Methylene chloride, acetonitrile, isopropyl alcohol, n-hexane, n-heptane, as well as the reagents used for preparing buffer solutions, were qualified as chemically pure or pure for analysis and were obtained from Chimmed (Moscow, Russia). Experiments were conducted using purified water obtained by a reverse osmosis UVOI 1812C6 system (MEDIANA FILTER, Moscow, Russia).