Hela cells, Vero cells and J774 cells were purchased from ATCC. Bone marrow-derived macrophages (BMDMs) were generated from hematopoietic stem cells isolated from CD45.1+ or CD45.2+ mice bone marrow, and maintained in DMEM (Corning Cellgro) containing 10% FBS (Thermo Scientific), 10% L929-cell conditioned media (containing M-CSF), 2 mM L-glutamine, 1 mM sodium pyruvate and antibiotics (penicillin/streptomycin) (Corning cellgro), at 37C in a 5% CO2 incubator. After 6-day culture, cells were either split for further expansion or frozen with FBS containing 10% DMSO in liquid nitrogen for later use (38 (link)).
Antibiotics penicillin streptomycin
Manufactured by Corning
Antibiotics (penicillin/streptomycin) are a class of antimicrobial compounds used in laboratory settings. These compounds inhibit the growth and reproduction of certain bacteria, making them essential for various applications such as cell culture and microbiology research.
Automatically generated - may contain errors
Lab products found in correlation
Hela cell, by American Type Culture Collection (1 mentions)
Vero cell, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
L glutamine, by Corning (1 mentions)
J774 cells, by American Type Culture Collection (1 mentions)
L929 cell conditioned media, by Corning (1 mentions)
Fetal bovine serum (fbs), by Omega Scientific (1 mentions)
2 protocols using antibiotics penicillin streptomycin
1
Generation of Bone Marrow-derived Macrophages
2
HeLa Cell Culture Protocol
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HeLa cells were purchased from ATCC (CCL-2).
Cells were cultured at 37 °C under 5% CO2 in phenol-red-
containing Dulbecco’s Modified Eagle Medium (DMEM; Gibco Life
Tech., cat. #11960–044) supplemented with 10% fetal bovine
serum (Omega Scientific, cat. #FB02) and with 1× concentrations
of nonessential amino acids (Gibco Life Tech., cat. #11140–050)
sodium pyruvate (Gibco Life Tech., cat. #11360–070),l -glutamine (Gibco Life Tech., cat. #35050–061), and the antibiotics
penicillin/streptomycin (Corning Cellgro, cat. #30–002-C1).
Cells were grown in T75 culture flasks and subcultured at ∼75–80%
confluency (every ∼3–4 days).
Cells were cultured at 37 °C under 5% CO2 in phenol-red-
containing Dulbecco’s Modified Eagle Medium (DMEM; Gibco Life
Tech., cat. #11960–044) supplemented with 10% fetal bovine
serum (Omega Scientific, cat. #FB02) and with 1× concentrations
of nonessential amino acids (Gibco Life Tech., cat. #11140–050)
sodium pyruvate (Gibco Life Tech., cat. #11360–070),
penicillin/streptomycin (Corning Cellgro, cat. #30–002-C1).
Cells were grown in T75 culture flasks and subcultured at ∼75–80%
confluency (every ∼3–4 days).
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