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Enliten atp bioluminescence detection kit

Manufactured by Promega
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Sourced in United States

The ENLITEN ATP Bioluminescence Detection Kit is a highly sensitive and rapid method for detecting and quantifying adenosine triphosphate (ATP) in biological samples. The kit utilizes the luciferase enzyme-catalyzed reaction to generate bioluminescent light, which is proportional to the amount of ATP present. This straightforward and reliable assay can be used to measure ATP levels in a variety of applications.

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Lab products found in correlation

Spectramax i3, by Molecular Devices (1 mentions) Spectramax i3 multi mode plate reader, by Molecular Devices (1 mentions)

Spelling variants of this product

ENLITEN ATP Assay System Bioluminescence Detection Kit (61 citations) ENLITEN (9 citations) ENLITEN-ATP kit (8 citations) ENLITEN kit (7 citations) ENLITEN® ATP Assay System Bioluminescence Detection Kit for ATP Measurement (7 citations) ATP bioluminescence detection kit (6 citations) ATP detection kit (3 citations)

2 protocols using enliten atp bioluminescence detection kit

1

ATP Bioluminescence Assay in Cell Lines

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The ENLITEN ATP Bioluminescence Detection Kit (FF2000; Promega, Madison, MI, USA) was used for measurement of ATP in cell culture supernatants. Briefly, 8,000 U2OS or MCA205 wild‐type cells per well were seeded in 96‐well plates. The following day, the cells were treated and 24 h later, the supernatant was collected and centrifuged. The supernatant was transferred to a white bottom plate, and the enzyme and substrate from the kit were added. ATP‐dependent substrate conversion was measured by assessing luminescence at 560 nm in a SpectraMax I3 multi‐mode plate reader (Molecular Devices).
Humeau J., Sauvat A., Cerrato G., Xie W., Loos F., Iannantuoni F., Bezu L., Lévesque S., Paillet J., Pol J., Leduc M., Zitvogel L., de Thé H., Kepp O, & Kroemer G. (2020). Inhibition of transcription by dactinomycin reveals a new characteristic of immunogenic cell stress. EMBO Molecular Medicine, 12(5), e11622.
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2

ATP Bioluminescence Assay in U2OS Cells

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Eight thousand U2OS wild-type cells per well were seeded in a 96-well plate. The next day, cells were treated for 24 hours. Then the supernatant was collected, centrifuged and transferred to a white bottom 96-well plate. Enzyme and substrate from ENLITEN ATP Bioluminescence Detection Kit (FF2000; Promega, Madison, Michigan, USA) were added. ATP-dependent substrate conversion was measured by luminescence at 560 nm with a Spectramax I3 multimode plate reader (Molecular Devices).
Bezu L., Wu Chuang A., Sauvat A., Humeau J., Xie W., Cerrato G., Liu P., Zhao L., Zhang S., Le Naour J., Pol J., van Endert P., Kepp O., Barlesi F, & Kroemer G. (2022). Local anesthetics elicit immune-dependent anticancer effects. Journal for Immunotherapy of Cancer, 10(4), e004151.
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