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The HEK293T/c17 is a cell line derived from human embryonic kidney cells. It is a subclone of the HEK293T cell line, which is commonly used in various research applications.

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Lab products found in correlation

Mirus transit lt1, by Mirus Bio (1 mentions) Polybrene, by Merck Group (1 mentions) Steriflip filter, by Merck Group (1 mentions) Pspax2, by Addgene (1 mentions) Pmd2 g, by Addgene (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thomas Scientific (1 mentions) Hct116, by American Type Culture Collection (1 mentions) Sw480, by American Type Culture Collection (1 mentions) Sw620, by American Type Culture Collection (1 mentions) Ht 29, by American Type Culture Collection (1 mentions)

2 protocols using hek293t c17

1

Lentiviral-mediated JunD knockdown

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To disrupt JunD expression, shRNAs from The RNAi Consortium (TRC) were screened for knockown potential in SW480 cells by transient transfection of purified plasmid using Mirus TransIT-LT1 (Mirus Bio, Madison, WI) followed by western blotting to monitor JunD expression (MAB5526 antibody; R&D Systems, Minneapolis, MN). TRC clone TRCN0000014975 was found to reduce JunD expression to the greatest extent (data not shown) and was used in subsequent experiments. Lentiviral plasmids were co-transfected with packaging vectors psPAX2 (Addgene plasmid #12260; a gift from Didier Trono) and pMD2.G (Addgene plasmid #12259; a gift from Didier Trono) into HEK293T/c17 cells (ATCC) using Mirus TransIT-Lenti. Virus-containing medium was collected 48 h after transfection and cleared of potential cells using 0.45-μm Steriflip filters (MilliporeSigma). Virus-containing media were mixed with Polybrene (MilliporeSigma) for transduction. Expression of JunD was assessed using the MAB5526 antibody. Scramble control shRNA on the same vector backbone (pLKO.1-puro non-target control shRNA; MilliporeSigma) was used as a control.
Ghaffari S., Hanson C., Schmidt R.E., Bouchonville K.J., Offer S.M, & Sinha S. (2021). An integrated multi-omics approach to identify regulatory mechanisms in cancer metastatic processes. Genome Biology, 22, 19.
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2

Cell Culture Maintenance Protocol

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Low-passage HEK293T/c17 (ATCC CRL-11268), Hela (ATCC CCL-2), HepG2 (ATCC HB-8065), HCT116 (ATCC CCL-247), RKO (ATCC CRL-2577), SW480 (ATCC CCL-228), SW620 (ATCC CCL-227) and HT-29 (ATCC HTB-38) cells were obtained from the American Type Culture Collection (ATCC) in 2013 and maintained at 37°C in a humidified incubator with an atmosphere of 5% CO2. Cells were cultured using DMEM (Mediatech) supplemented with 10% FBS (Denville Scientific), penicillin and streptomycin (Mediatech).
Wu R., Nie Q., Tapper E.E., Jerde C.R., Dunlap G.S., Shrestha S., Elraiyah T.A., Offer S.M, & Diasio R.B. (2016). Histone H3K27 trimethylation modulates 5-fluorouracil resistance by inhibiting PU.1 binding to the DPYD promoter. Cancer research, 76(21), 6362-6373.
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