Fluo 4 direct ca2 channel test kit

According to the manufacturer’s instructions, the Fluo-4 Direct Ca²⁺ channel test kit was used to examine the effect of SMJ-5 on human calcium channels (Life Technologies, Carlsbad, CA, USA) (23 (link)). HEK-293T cells (5 × 10⁴) were treated with Probenecid (5 mM) and Fluo-4 dye for 1 h, and the amount of fluorescence was measured. Then the cells were treated with indole derivatives at 1/4× MIC, DMSO (vehicle control), and verapamil at 50 µg/mL. Carbachol (50 µg/mL), the Ca²⁺ channel stimulator, was added after 2 min, and the fluorescence was recorded at 494/516 nm using a microplate reader.

According to the manufacturer’s instructions, the Fluo-4 Direct Ca²⁺ channel test kit was used to examine the effect of SMJ-5 on human calcium channels (Life Technologies, Carlsbad, CA, USA) (23 (link)). HEK-293T cells (5 × 10⁴) were treated with Probenecid (5 mM) and Fluo-4 dye for 1 h, and the amount of fluorescence was measured. Then the cells were treated with indole derivatives at 1/4× MIC, DMSO (vehicle control), and verapamil at 50 µg/mL. Carbachol (50 µg/mL), the Ca²⁺ channel stimulator, was added after 2 min, and the fluorescence was recorded at 494/516 nm using a microplate reader.