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Anti cd43 magnetic microbeads

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Anti-CD43 magnetic microbeads are laboratory reagents used for cell separation and enrichment. These microbeads are coated with antibodies that specifically bind to the CD43 surface antigen, enabling the isolation or depletion of CD43-positive cells from complex samples.

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Lab products found in correlation

Lipopolysaccharides (lps), by Merck Group (1 mentions)

Spelling variants of this product

Magnetic microbeads (147 citations) CD43 microbeads (41 citations) Anti-CD43 MicroBeads (35 citations) CD43-magnetic microbeads (3 citations) Anti-CD43 (2 citations)

3 protocols using anti cd43 magnetic microbeads

1

Isolation and Culture of Murine B Cells

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Untouched B cells were separated from total spleen cell suspensions using anti-CD43 magnetic microbeads (Miltenyi). The purity of isolated untouched B cell was typically around 96%–98%. Untouched B cells were cultured at a concentration of 5 × 105 cells/ml in RPMI medium supplemented with 15% FBS, penicillin-streptomycin (100 units/ml) and L-glutamine (2 mM).
Jiang C., Trudeau S.J., Cheong T.C., Guo R., Teng M., Wang L.W., Wang Z., Pighi C., Gautier-Courteille C., Ma Y., Jiang S., Wang C., Zhao B., Paillard L., Doench J.G., Chiarle R, & Gewurz B.E. (2019). CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation. Cell reports, 28(5), 1307-1322.e8.
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2

Retroviral Transduction of Murine B Cells

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Splenocytes from 8- to 10-week-old female C57BL/6 mice (in house breeding) or corresponding B-cell-specific grb2-ko (Grb2fl/flmb1cre/+) mice26 (link) were depleted of CD43-positive cells using anti-CD43 magnetic microbeads (Miltenyi Biotec). The remaining cells, which were >90% positive for CD19 and IgM, were incubated for 24 h with 10 μg per ml LPS (Sigma) in RPMI1640+Glutamaxx supplemented with 10% heat-inactivated FCS and antibiotics. Subsequently 2 × 106 cells were infected with retrovirus-containing supernatants from Plat-E packaging cells supplemented with 3 μg ml−1 polybrene. Cells were used for assays 24 to 48 h after infection.
Engels N., König L.M., Schulze W., Radtke D., Vanshylla K., Lutz J., Winkler T.H., Nitschke L, & Wienands J. (2014). The immunoglobulin tail tyrosine motif upgrades memory-type BCRs by incorporating a Grb2-Btk signalling module. Nature Communications, 5, 5456.
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3

Isolation and Culture of Murine B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Untouched B cells were separated from total spleen cell suspensions using anti-CD43 magnetic microbeads (Miltenyi). The purity of isolated untouched B cell was typically around 96%–98%. Untouched B cells were cultured at a concentration of 5 × 105 cells/ml in RPMI medium supplemented with 15% FBS, penicillin-streptomycin (100 units/ml) and L-glutamine (2 mM).
Jiang C., Trudeau S.J., Cheong T.C., Guo R., Teng M., Wang L.W., Wang Z., Pighi C., Gautier-Courteille C., Ma Y., Jiang S., Wang C., Zhao B., Paillard L., Doench J.G., Chiarle R, & Gewurz B.E. (2019). CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation. Cell reports, 28(5), 1307-1322.e8.
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