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Afuresertib

Manufactured by Cayman Chemical
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Sourced in United States

Afuresertib is a selective and potent inhibitor of the protein kinase AKT. It is used for research purposes in the laboratory setting.

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Lab products found in correlation

Vo ohpic, by Bio-Techne (1 mentions) Amphotericin, by Thermo Fisher Scientific (1 mentions) Estradiol, by R&D Systems (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Mtt cell proliferation assay, by American Type Culture Collection (1 mentions) Caco 2 cell, by American Type Culture Collection (1 mentions) Deadend fluorometric tunel assay kit, by Promega (1 mentions) Gw3965, by R&D Systems (1 mentions) Dnase, by Thermo Fisher Scientific (1 mentions) Sw620 cells, by American Type Culture Collection (1 mentions) Immun blot pvdf membrane for protein blotting, by Bio-Rad (1 mentions) Hard set mounting medium with dapi, by Vector Laboratories (1 mentions) Leibovitz s l 15 media, by Thermo Fisher Scientific (1 mentions) Cytotox 96 non radioactive cytotoxicity assay kit, by Promega (1 mentions) Dmem media, by Thermo Fisher Scientific (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)

2 protocols using afuresertib

1

Afuresertib and VO-OHpic Small-Molecule Inhibitors

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Afuresertib was obtained from Cayman Chemical (Ann Arbor, MI, United States). VO-OHpic was obtained from Tocris (Minneapolis, MN, United States). The small-molecule inhibitors dissolved in DMSO as stock solutions were diluted in media and applied to COs during the time period illustrated schematically in Figure 9A.
Lin V.J., Hu J., Zolekar A., Yan L.J, & Wang Y.C. (2020). Urine Sample-Derived Cerebral Organoids Suitable for Studying Neurodevelopment and Pharmacological Responses. Frontiers in Cell and Developmental Biology, 8, 304.
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2

Caco2 and SW620 Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caco2 cells (cat # HTB-37™), MTT Cell Proliferation Assay (cat # 30–1010K), and SW620 cells (cat # CCL-227™) (ATCC, Manassas, VA). DMEM media, Leibovitz’s L-15 media, 100U/ml penicillin, 100ug/ml streptomycin, and 0.25ug/ml amphotericin (Life Technologies, Carlsbad, CA). FBS (Atlanta Biologicals, Flowery Branch, GA). GW3965 (cat # 2474), 27-hydroxycholesterol (cat # 3907), and estradiol (cat # 2824) (R&D systems from Minneapolis, MN). 6α-epoxycholesterol-3-sulfate (cat # C4136–000) (Steraloids, Newport, RI). Afuresertib (cat # 17988) (Cayman Chemicals from Ann Arbor, MI). CytoTox 96® non-radioactive cytotoxicity assay kit (cat # G1782) and DeadEnd Fluorometric TUNEL assay kit (cat # G3250) (Promega, Valencia, CA). Hard Set mounting medium with DAPI (cat # H-1500) (Vector Laboratories, Burlingame, CA). DNase (cat #AM2222), Alexa Fluor 594nm (cat #A11037), Halt™ proteases and phosphatase inhibitor cocktail (cat #78446) (Fisher Scientific, Hampton, NH). Immun-Blot® PVDF membrane for protein blotting (cat # 1620177), Clarity™ Western ECL substrate (BioRAD, Hercules, CA). The antibodies used in this study are included in Table 1 below.
Warns J., Marwarha G., Freking N, & Ghribi O. (2018). 27-hydroxycholesterol decreases cell proliferation in colon cancer cell lines. Biochimie, 153, 171-180.
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