Cells in the logarithmic growth phase were digested with 0.1% trypsin/0.1% EDTA into single cell suspensions. Cells were respectively labeled with rabbit anti-human nestin (Millipore, Germany), Oct4 (Cell Signaling, USA), vimentin (Abcam, UK), NANOG (Abcam), and CD90 (Abcam) antibodies, and mouse anti-human CD105 (Abcam) antibody. About 1 × 106 cells were used for detection of each molecule. Alexa Fluor 488-conjugated anti-mouse and anti-rabbit were used as secondary antibodies. Labeled cells were thoroughly washed followed by centrifugation three times, and then resuspended in PBS solution. Flow cytometry (EPICS XL; Beckman, USA) was used to detect fluorescence intensity and the number of positive cells.
Mouse anti human cd105
Manufactured by Abcam
Sourced in United Kingdom
Mouse anti-human CD105 is a monoclonal antibody that recognizes the human CD105 antigen. CD105, also known as endoglin, is a cell surface glycoprotein that is highly expressed on endothelial cells and is involved in angiogenesis and cell proliferation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse anti human cd105
1
Multimarker Characterization of Stem Cells
2
Characterization of Cultured MSCs
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