2545 quaternary gradient module

Manufactured by Waters Corporation

Sourced in United States

The 2545 Quaternary Gradient Module is a core component of Waters Corporation's liquid chromatography systems. It is designed to precisely mix and deliver four independent solvent streams at a controlled flow rate, enabling the creation of complex solvent gradients for advanced chromatographic separations.

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Lab products found in correlation

2767 sample manager, by Waters Corporation (2 mentions) 2998 photodiode array detector, by Waters Corporation (2 mentions) 3100 mass detector, by Waters Corporation (1 mentions) Hypercarb, by Thermo Fisher Scientific (1 mentions) Acquity upc2, by Waters Corporation (1 mentions) Fraction collector 3, by Waters Corporation (1 mentions) Prep lc system, by Waters Corporation (1 mentions)

Close spelling variants of this product

Waters 2545 quaternary gradient module 2545 Quaternary Gradient Module pump

3 protocols using 2545 quaternary gradient module

Preparative Fractionation of Reduced Glycans

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Fractionation
was performed on a Waters preparative LC–MS system, equipped
with a 2767 sample manager, 2545 quaternary gradient module, fluid
organizer, 3100 mass detector, and 2998 photodiode array detector
(Waters, Milford, MA, U.S.A.). Reduced (including non-reducing) GOS
DP3 (95 mg/mL, 0.5 mL) was centrifuged (5 min, 15000g) and injected onto a PGC column (150 × 21.2 mm, 5 μm
particle size, Hypercarb; Thermo Scientific, San Jose, CA, U.S.A.).
The flow rate was set at 18.34 mL/min. Mobile phase A consisted of
ULC–MS water + 0.1% (v/v) formic acid, and mobile phase B consisted
of ACN + 0.1% (v/v) formic acid. The gradient applied was as follows:
0–80.8 min, 3–11% B; 80.8–95.3 min, 11–100%
B; and 95.3–109.9 min, 100–3% B, followed by equilibration
at 3% B. Fractions of 6.1 mL were collected and analyzed using analytical
UHPLC–PGC–MS (see section 2.3.2). Fractions were pooled on the basis
of the retention time and MS² fragmentation of the isomer
present. Only fractions with a purity of 90% according to the signal
intensity as measured by analytical UHPLC–PGC–MS were
pooled. Afterward, ACN was evaporated under a stream of nitrogen gas
and the remaining water phase was freeze-dried.

Logtenberg M.J., Donners K.M., Vink J.C., van Leeuwen S.S., de Waard P., de Vos P, & Schols H.A. (2020). Touching the High Complexity of Prebiotic Vivinal Galacto-oligosaccharides Using Porous Graphitic Carbon Ultra-High-Performance Liquid Chromatography Coupled to Mass Spectrometry. Journal of Agricultural and Food Chemistry, 68(29), 7800-7808.

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Chiral Resolution of 8-Fluoro-Quinoline Derivative

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Example 3

The racemic 8-fluoro-N-(1-isopropyl-1-methyl-3-phenyl-prop-2-ynyl)quinoline-3-carboxamide mixture was submitted to chiral resolution by preparative HPLC chromatography using the conditions outlined hereafter to deliver (S)-8-fluoro-N-(1-isopropyl-1-methyl-3-phenyl-prop-2-ynyl)quinoline-3-carboxamide and (R)-8-fluoro-N-(1-isopropyl-1-methyl-3-phenyl-prop-2-ynyl)quinoline-3-carboxamide.

Analytical HPLC Method:

SFC:

Waters Acquity UPC²/QDa

PDA Detector Waters Acquity UPC²

Column: Daicel SFC CHIRALPAK® IF, 3 □m, 0.3 cm×10 cm, 40° C.

Mobile phase: A: CO₂B: MeOH gradient: 20-40% B in 1.8 min

ABPR: 1800 psi

Flow rate: 2.0 ml/min

Detection: 240 nm

Sample concentration: 1 mg/mL in Hept/iPr 50/50

Injection: 1 μL

Preparative HPLC Method:

Autopurification System from Waters: 2767 sample Manager, 2489 UVNisible Detector,

2545 Quaternary Gradient Module.

Column: Daicel CHIRALPAK® IF, 5 μm, 1.0 cm×25 cm

Mobile phase: Hept/EtOH 95/05

Flow rate: 10 ml/min

Detection: UV 240 nm

Sample concentration: 60 mg/mL in/EtOAc

Injection: 80-160 μl

First eluting enantiomerSecond eluting enantiomer

Retention time (min) ~1.23Retention time (min) ~1.52

Chemical purity (area %Chemical purity (area %

at 240 nm) 99at 240 nm) 99

Enantiomeric excess (%) >99Enantiomeric excess (%) >99

US11178869B2. Microbiocidal quinoline (thio)carboxamide derivatives (2021-11-23). SYNGENTA PARTICIPATIONS AG [CH]. Inventors: Laura Quaranta [CH], Matthias Weiss [CH], Farhan Bou Hamdan [CH].

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Extraction and Isolation of L. tanakae Compounds

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L. tanakae (1.032 kg) was refluxed with 6 L of 70% ethanol for 2 h twice. The extraction filtered through chromatography paper (46 × 57 cm) and removed the solvent in vacuo. The yield of the L. tanakae 70% ethanol extract (LTE) was 126.42 g (12.25%, w/w) and it was stored at −20 °C. Four of the major compounds were isolated using preparative high performance liquid chromatography (HPLC, Prep LC) from the 70% ethanol extract. A 100 mg aliquot of LTE was dissolved in 70% ethanol (1 mL) and filtered through a syringe filter (0.45 μm) before injecting it into the HPLC system. The Prep LC system (Waters, Milford, MA, USA) consisted of a Waters 2545 quaternary gradient module, Waters 2998 photodiode array detector, Waters flexinject, and Waters fraction collector III. Four components were isolated using a Phenomenex Synergi 4μ Fusion-RP 80A (250 × 21.20 mm, Phenomenex Inc., Torrance, CA, USA). The mobile phase was prepared by mixing 0.05% aqueous formic acid (A) and acetonitrile (B), and set in a linear gradient program; 5% A → 30% A for 60 min. The flow rate was 5 mL/min and injected volume was 500 μL at room temperature and detection was conducted at a wavelength of 254 nm. Chemical structures were determined with spectroscopic methods using mass spectroscopy and NMR.

Park S.W., Lee A.Y., Lim J.O., Lee S.J., Kim W.I., Yang Y.G., Kim B., Kim J.S., Chae S.W., Na K., Seo Y.S, & Shin I.S. (2022). Loranthus tanakae Franch. & Sav. Suppresses Inflammatory Response in Cigarette Smoke Condensate Exposed Bronchial Epithelial Cells and Mice. Antioxidants, 11(10), 1885.

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