Anti phospho akt

The fruiting body of Hericium erinaceus was purchased from Jilin Jiaohe Songshan Food Co., Ltd. (Jiaohe, China). Dextran standards (670, 410, 270, 80, 25, 12 and 5 kDa) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Monosaccharide standards, including arabinose, mannose, fucose, xylose, rhamnose, galactose and glucose were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Cell Counting Kit-8 was obtained from APExBIO Technology LLC (Houston, TX, USA). Neutral red staining solution, nitric oxide assay kit and BCA kit were obtained from Beyotime Biological Technology Co., Ltd. (Shanghai, China). Western Blot assay kit, SDS-PAGE kit, ELISA kits of TNF-α, IL-6, IL-10 and IFN were obtained from Boster Biological Technology Co., Ltd. (Wuhan, China). Anti-ERK, anti-phospho ERK, anti-JNK, anti-phospho JNK, anti-p38 MAPK, anti-phospho p38 MAPK, anti-Akt, anti-phospho Akt, anti-IkBα, anti-p65, anti-β-actin and anti-Histone H2A were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China). Inhibitors of BAY11-7082, SB203580, SP600125, PD98059 and LY294002 were purchased from Selleck Chemicals (Shanghai, China).