Log In Sign up
The largest database of trusted experimental protocols

Nunc lab tek 2 8 well chamber slide system

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The Nunc™ Lab-Tek™ II 8 well chamber slide system is a laboratory equipment designed for cell culture and microscopic observation. It provides a convenient platform for culturing and analyzing cells in a controlled environment.

Automatically generated - may contain errors

Lab products found in correlation

Lsm780 confocal laser scanning microscope, by Zeiss (3 mentions) Anti gfap rabbit monoclonal antibody, by Cell Signaling Technology (2 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (2 mentions) Leucocyte acid phosphatase kit, by Merck Group (2 mentions) Murine rankl, by R&D Systems (2 mentions) Rankl, by Thermo Fisher Scientific (2 mentions) Adiporon, by Merck Group (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) β mercaptoethanol, by Thermo Fisher Scientific (2 mentions) Triton x x100, by Merck Group (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Imaris v9, by Oxford Instruments (1 mentions)

Close spelling variants of this product

Lab-Tek chamber slides (385 citations) Chamber slides (251 citations) Lab-Tek (191 citations) Lab-Tek II (175 citations) Nunc Lab-Tek (110 citations) Lab-Tek chambers (110 citations) Nunc Lab-Tek II (60 citations) NuncTM (46 citations) Lab-Tek II chamber (24 citations) 8-chamber slides (22 citations)

5 protocols using nunc lab tek 2 8 well chamber slide system

1

Immunofluorescence Staining of GFAP

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in Nunc Lab-Tek II Chamber slide system (8-well) (ThermoFischer Scientific). Slides were fixed with 4% paraformaldehyde in 2% bovine serum albumin (BSA) for 15 min at room temperature, followed by washing three times with PBS. Cells were permeabilized by 0.05% Triton X-100, 2% BSA, 5% normal goat serum (NGS) in PBS followed by 3 PBS washes. Slides were blocked with 2% BSA, 5% NGS in PBS for 1 hour, followed by overnight incubation with anti-GFAP rabbit monoclonal antibody (Cell Signalling #12389) at 1:200 dilution in blocking solution. Cells were washed 3 times with PBS and incubated for 1 hour with 1:500 dilution of Goat anti-rabbit IgG cross-adsorbed secondary antibody, Alexa Fluor 488 (ThermoFischer Scientific) in blocking solution. Slides were washed 3 times in PBS and Prolong Gold antifade reagent with DAPI (Invitrogen) was applied. Slides were photographed with Zeiss LSM780 Laser Scanning Confocal Microscope at 20X and 63X magnification.
Jessa S., Blanchet-Cohen A., Krug B., Vladoiu M., Coutelier M., Faury D., Poreau B., De Jay N., Hébert S., Monlong J., Farmer W.T., Donovan L.K., Hu Y., McConechy M.K., Cavalli F.M., Mikael L.G., Ellezam B., Richer M., Allaire A., Weil A.G., Atkinson J., Farmer J.P., Dudley R.W., Larouche V., Crevier L., Albrecht S., Filbin M.G., Sartelet H., Lutz P.E., Nagy C., Turecki G., Costantino S., Dirks P.B., Murai K.K., Bourque G., Ragoussis J., Garzia L., Taylor M.D., Jabado N, & Kleinman C.L. (2019). Stalled developmental programs at the root of pediatric brain tumors. Nature genetics, 51(12), 1702-1713.
+ Open protocol
+ Expand
2

Immunofluorescence Staining of GFAP

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in Nunc Lab-Tek II Chamber slide system (8-well) (ThermoFischer Scientific). Slides were fixed with 4% paraformaldehyde in 2% bovine serum albumin (BSA) for 15 min at room temperature, followed by washing three times with PBS. Cells were permeabilized by 0.05% Triton X-100, 2% BSA, 5% normal goat serum (NGS) in PBS followed by 3 PBS washes. Slides were blocked with 2% BSA, 5% NGS in PBS for 1 hour, followed by overnight incubation with anti-GFAP rabbit monoclonal antibody (Cell Signalling #12389) at 1:200 dilution in blocking solution. Cells were washed 3 times with PBS and incubated for 1 hour with 1:500 dilution of Goat anti-rabbit IgG cross-adsorbed secondary antibody, Alexa Fluor 488 (ThermoFischer Scientific) in blocking solution. Slides were washed 3 times in PBS and Prolong Gold antifade reagent with DAPI (Invitrogen) was applied. Slides were photographed with Zeiss LSM780 Laser Scanning Confocal Microscope at 20X and 63X magnification.
Jessa S., Blanchet-Cohen A., Krug B., Vladoiu M., Coutelier M., Faury D., Poreau B., De Jay N., Hébert S., Monlong J., Farmer W.T., Donovan L.K., Hu Y., McConechy M.K., Cavalli F.M., Mikael L.G., Ellezam B., Richer M., Allaire A., Weil A.G., Atkinson J., Farmer J.P., Dudley R.W., Larouche V., Crevier L., Albrecht S., Filbin M.G., Sartelet H., Lutz P.E., Nagy C., Turecki G., Costantino S., Dirks P.B., Murai K.K., Bourque G., Ragoussis J., Garzia L., Taylor M.D., Jabado N, & Kleinman C.L. (2019). Stalled developmental programs at the root of pediatric brain tumors. Nature genetics, 51(12), 1702-1713.
+ Open protocol
+ Expand
3

Fluorescent Micelle Visualization in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
To obtain fluorescent micelles, DiD was dissolved in macrophage media to 5 µM and pluronic L101 added to a final concentration of 0.01% (w/v). RAW 264.7 and THP-1 cells (seeded at 2 × 104 cell per well in a Nunc™ Lab-Tek™ II 8 well chamber slide system, 12-565-8, Thermo Scientific, Waltham, MA, USA) were treated with DiD-loaded micelles, or with DiD alone for 30 min. The cell membrane was stained using MemBrite® Fix Staining (30095, Biotium, Fremont, CA, USA) following the manufacturer’s instructions. Cells were fixed in 2% paraformaldehyde, and permeabilized with 0.1% Triton-X (X100; Sigma-Aldrich, St. Louis, MO, USA). Cells were then stained with 0.0012 µM DAPI (D3571; Invitrogen, Carlsbad, CA, USA) and mounted with ProLong™ Gold Antifade Mountant (P36930, Thermo Scientific, Waltham, MA, USA). Three-dimensional image acquisition was done using a LSM 780 laser scanning confocal microscope (Carl Zeiss Microscopy, Jena, Germany). Three-dimensional reconstructions were carried out using Imaris v9.5.0 (Bitplane AG, Zürich, Switzerland).
Cartaya A.E., Lutz H., Maiocchi S., Nalesnik M, & Bahnson E.M. (2021). Delivery of Cinnamic Aldehyde Antioxidant Response Activating nanoParticles (ARAPas) for Vascular Applications. Antioxidants, 10(5), 709.
+ Open protocol
+ Expand
4

Osteoclastogenesis Modulation by AdipoRon

Check if the same lab product or an alternative is used in the 5 most similar protocols
For osteoclastogenesis, a total of 1 × 104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco) as well as 50 μM β-mercaptoethanol (Gibco) and 30 ng/ml murine RANKL (R&D Systems). To study the impact of AdipoRon on osteoclast differentiation, 10 μM AdipoRon (Sigma-Aldrich) were added to the culture medium. Tartrate-resistant acid phosphatase (TRAcP) activity was examined using the leucocyte acid phosphatase kit (Sigma-Aldrich) according to manufacturer’s specifications. TRAcP+ cells with ≥3 nuclei were considered as osteoclasts. In addition, 5 × 103 RAW 264.7 cells were seeded per well in a Nunc Lab-Tek II 8-well Chamber Slide system (ThermoFisher Scientific) in a total of 300 μl osteoclast differentiation medium containing 30 ng/ml RANKL. Fully differentiated osteoclasts were treated with 10 μM AdipoRon for the indicated time points. FACS analysis as well as sorting of mature OCLs for subsequent Western-blot analyses was performed as described previously (Madel et al., 2018 ).
Madel M.B., Fu H., Pierroz D.D., Schiffrin M., Winkler C., Wilson A., Pochon C., Toffoli B., Taïeb M., Jouzeau J.Y., Gilardi F., Ferrari S., Bonnet N., Blin-Wakkach C., Desvergne B, & Moulin D. (2021). Lack of Adiponectin Drives Hyperosteoclastogenesis in Lipoatrophic Mice. Frontiers in Cell and Developmental Biology, 9, 627153.
+ Open protocol
+ Expand
5

Osteoclastogenesis Protocol with AdipoRon

Check if the same lab product or an alternative is used in the 5 most similar protocols
For osteoclastogenesis, a total of 1x10 4 RAW 264.7 (ATCC) cells were seeded per well on 24well plates in 500 µl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillinstreptomycin (Gibco) as well as 50 μM β-mercaptoethanol (Gibco) and 30 ng/ml murine RANKL (R&D Systems). To study the impact of AdipoRon on osteoclast differentiation, 10 µM AdipoRon (Sigma-Aldrich) were added to the culture medium. Tartrate-resistant acid phosphatase (TRAcP) activity was examined using the leucocyte acid phosphatase kit (Sigma-Aldrich) according to manufacturer's specifications. TRAcP + cells with ≥3 nuclei were considered as osteoclasts. In addition, 5x10 3 RAW 264.7 cells were seeded per well in a Nunc
Lab-Tek II 8-well Chamber Slide system (ThermoFisher Scientific) in a total of 300 µl osteoclast differentiation medium containing 30 ng/ml RANKL. Fully differentiated osteoclasts were treated with 10 µM AdipoRon for the indicated time points. FACS analysis as well as sorting of mature OCLs for subsequent Western-blot analyses was performed as described previously (Madel et al., 2018) (link).
Fu H., Madel M., Pierroz D.D., Schiffrin M.J., Winkler C., Wilson A., Pochon C., Toffoli B., Jouzeau J., Gilardi F., Ferrari S., Bonnet N., Blin‐Wakkach C., Desvergne B., & Moulin D. (2020). Adipose-tissue derived signals control bone remodelling.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!