For in vivo real-time biodistribution imaging of SBC-EV(ICG/PTX) and free ICG, MCF-7 tumor-xenograft mice were intravenously injected with 100 μL of free ICG and SBC-EV(ICG/PTX) (1.0 mg ICG/kg and 0.4 mg PTX/kg). The in vivo biodistribution imaging was carried out using an IVIS system (Ami HT imaging system, Spectral Instruments Imaging, Tucson, AZ, USA) by measuring the ICG fluorescence intensity of whole body with a long wave emission filter (745-830 nm) at predetermined time intervals within 24 h. The mice were euthanized at 4 h or 24 h-post injection to obtain ex vivo fluorescent images of major organs and tumors of the mice.
Ami ht imaging system
Manufactured by Spectral Instruments Imaging
Sourced in United States
The Ami HT imaging system is a high-throughput microscope designed for rapid image acquisition and analysis. It features a motorized stage, autofocus capabilities, and a high-speed camera for efficient data collection. The core function of the Ami HT is to provide researchers with a versatile imaging platform for a variety of applications.
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Lab products found in correlation
3 protocols using ami ht imaging system
1
Real-Time Biodistribution Imaging of SBC-EV(ICG/PTX)
2
Real-Time Biodistribution of Photosensitizers in Glioblastoma
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To evaluate in vivo real-time biodistribution of bEV–(TPP-Ce6), Ce6, and TPP-Ce6, orthotopic GBM-xenografted mice (n = 4) received the intravenous (i.v.) injection of each sample (100 μL, 1.71 μmol/kg for TPP-Ce6). Whole-body fluorescence images were acquired at the predetermined time intervals within 24 h after i.v. injection using in vivo imaging systems (IVIS) with a long wavelength emission filter (640–720 nm) (Ami HT imaging system, Spectral Instruments Imaging, Tucson, AZ, USA). Quantitative analysis of fluorescence intensity in the images was carried out using the region of interest (ROI) function of imaging software (Aura Imaging 2.20, Spectral Instruments Imaging). The ROI of the brain tumor was manually selected based on its bioluminescence.
3
Bioluminescence Imaging in Mice
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Bioluminescence imaging was performed using an Ami HT imaging system (Spectral Instruments Imaging) and analysis software (Aura imaging). Briefly, mice were injected with 150 mg/kg D-luciferin (PerkinElmer) intraperitoneally and placed in a chamber for isoflurane anesthesia. Mice were then placed in the Ami HT imaging system and monitored for bioluminescence within 10 min after D-luciferin injection.
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