Antibodies used for phenotyping include: CD24-PE (clone M1/69 eBioscience Inc. San Diego CA), CD34-FITC (clone 1H6 Serotech, Raleigh, NC), CD44-FITC (clone 1M7 eBioscience Inc. San Diego CA), CD45-FITC (clone YKIX716.13 Serotech, Raleigh, NC), CD73-biotin (clone TY/11.8, Biolegend. San Diego CA), CD90-APC (clone YKIX337.217, eBioscience Inc., San Diego CA), Oct3/4 (clone EM92, eBioscience Inc. San Diego CA), and unconjugated CD105 (clone 8A1, Abcam. Cambridge, MA).
Cd73 biotin clone ty 11
Manufactured by BioLegend
CD73-biotin (clone TY/11.8) is a biotinylated antibody that recognizes the CD73 protein. CD73 is an enzyme involved in the conversion of extracellular adenosine monophosphate (AMP) to adenosine. This antibody can be used for the detection and isolation of cells expressing CD73.
Automatically generated - may contain errors
Lab products found in correlation
Cd44 fitc, by Thermo Fisher Scientific (1 mentions)
Cd24 pe clone m1 69, by Thermo Fisher Scientific (1 mentions)
Streptavidin bv421, by BioLegend (1 mentions)
Igg1 fitc, by BD (1 mentions)
Anti human iga, by Agilent Technologies (1 mentions)
Pe cy7 cd105, by BioLegend (1 mentions)
Ki67 pe cy7, by BD (1 mentions)
Cd31 pe, by BioLegend (1 mentions)
Anti mouse iga ab pe, by Abcam (1 mentions)
Pe anti mouse cd138, by BioLegend (1 mentions)
Anti rabbit igg bv421, by BioLegend (1 mentions)
Cd45 percp cy5, by BioLegend (1 mentions)
B220 apc cy7, by BioLegend (1 mentions)
2 protocols using cd73 biotin clone ty 11
1
Mesenchymal Stem Cell Phenotyping
2
Immunostaining and Flow Cytometry Protocols
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The following Abs were used for IF and IHC staining: anti-mouse IgA Ab-PE (Abcam, 97013) or fluorescein isothiocyanate (FITC; Abcam, 97234), anti-mouse CD138-PE (clone 281-2, BioLegend), anti-human IgA (DAKO, #IR51061), or CD138 (DAKO, M7228). For flow cytometry, the following Abs against mouse antigens were used: B220–APC-Cy7 (clone RA3-6B2, BioLegend), CD138-PE or BV421 (clone 281-2), GL7-PerCP Cy5.5 (clone GL7, BioLegend), Ki67–PE-Cy7 (clone B56, BD), IgG1-FITC (clone A85-1, BD), IgG2a/c-FITC (clone RMG2a-62, BioLegend), IgG2b-FITC (SouthernBiotech, 1092-02), IgG3-FITC (SouthernBiotech, 1102-02), IgA-biotin (clone RMA-1, BioLegend), CD73-biotin (clone TY/11.8, BioLegend), CD140b-APC (clone APB5, BioLegend), CD105–PE-Cy7 (clone MJ7/18, BioLegend), CD45–PerCP-Cy5.5 (clone 30-F11, BioLegend), and CD31-PE (clone 390, BioLegend); other reagents were also used: rabbit polyclonal anti-SPTBN1 (amino acids 2100 to 2150; Abcam, 72239), normal rabbit IgG (an isotype-matched control for the latter; Sigma-Aldrich, 12-370), anti-rabbit IgG-BV421 (clone Poly4064, BioLegend), streptavidin-BV421 (BioLegend, 405226), or APC (BioLegend, 405207).
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