Vitamin e d alpha tocopherol polyethylene glycol 1000 succinate tpgs

GTX (sesquihydrate) was obtained from ThermoFisher Scientific (Hanover Park, IL USA). Glycerin and Tween^® 80 were obtained from Spectrum Pharmaceuticals (Henderson, NV, USA). Geleol^TM and Precirol^® ATO 5 were gifted by Gatefossé. Amicon^® ultra centrifugal filter devices, centrifuge tubes (1.5 mL), glass scintillation vials, HPLC vials, Slide-A-Lyzer™ MINI dialysis devices, vitamin E-d-alpha-tocopherol polyethylene glycol 1000 succinate (TPGS), and all other chemicals were obtained from Fisher Scientific Hampton, NH, USA). High-performance liquid chromatography (HPLC) grade solvents were used for analysis. Microbial strains of methicillin-resistant Staphylococcus aureus (ATCC 1708) and Pseudomonas aeruginosa (BAA-2018) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cation-adjusted Mueller Hinton Broth 2 was obtained from MilliporeSigma (St. Louis, MO, USA). Pel-Freez Biologicals (Rogers, AR, USA) supplied whole eyes of albino rabbits (New Zealand) shipped overnight.

Cannabidiol (CBD) derived from hemp was used in this study and was a gift from ElSohly Laboratories, Inc. (Oxford, MS, USA). Optical rotation, melting point test, mass spectroscopy, and nuclear magnetic resonance spectroscopy studies were performed to ensure that this investigation was carried out with the (-) normal CBD isomer. Poloxamer 407 NF grade, sesame oil NF grade, Carbopol^® 940 NF grade, glycerin NF grade, and Tween^® 80 (Polysorbate 80) NF grade were purchased from Spectrum Chemicals (New Brunswick, NJ, USA). All other chemicals, including Vitamin E-d-alpha-tocopherol polyethylene glycol 1000 succinate (TPGS), were purchased from Fisher Scientific (St. Louis, MO, USA). Solvents used for the instrumental analysis were of high-performance liquid chromatography (HPLC) grade and were purchased from Fischer Scientific (St. Louis, MO, USA). Centrifuge tubes, scintillation glass vials, and Slide-A-Lyzer™ MINI Dialysis Devices (10 K molecular weight cutoff) were purchased from Fischer Scientific (Hampton, NH, USA). Screw top clear class A (Type I) borosilicate glass HPLC vials (12 × 32 mm, 2.0 mL) with pre-slit silicone septa were purchased from Waters (Waters, Milford, CA, USA).