Zeb2 sip1

Manufactured by Santa Cruz Biotechnology

ZEB2/SIP1 is a protein involved in the regulation of gene expression. It functions as a transcriptional repressor that plays a role in various cellular processes. The core function of ZEB2/SIP1 is to modulate the expression of target genes, though its specific biological roles may vary depending on the cellular context.

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Lab products found in correlation

Vimentin, by Santa Cruz Biotechnology (2 mentions) E cadherin, by BD (2 mentions) Mucin 2, by Santa Cruz Biotechnology (1 mentions) A11037, by Thermo Fisher Scientific (1 mentions) A11059, by Thermo Fisher Scientific (1 mentions) α sma, by Abcam (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Myc tag, by Merck Group (1 mentions) β catenin, by BD (1 mentions) β actin, by Abcam (1 mentions)

2 protocols using zeb2 sip1

Murine Intestinal Organoid Staining

Cited 1 time

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Murine intestines were prepared as described previously^{5 (link)}. In situ hybridisation (ISH) assay was carried out as described previously^{5 (link)}. Organoids were immuno-stained either as whole-mount samples or as paraffin sections. Samples for IHC were processed as outlined before^{5 (link),24} and the following primary antibodies were used: ZEB2/SIP1 (H260; Santa-Cruz, or from Dr. Tulchinsky), E-cadherin (610181; BD), Vimentin (RV202; Santa-Cruz), α-SMA (ab5694; Abcam), Ki-67 (M7249; Dako) and Mucin2 (H300; Santa-Cruz). For IF, samples were exposed to goat anti-rabbit antibodies conjugated to Alexa Fluor594 (A11037; Invitrogen) and/or rabbit anti-mouse antibodies conjugated to Alexa Fluor488 (A11059; Invitrogen). Tetramethylrhodamine-B isothiocyanate (TRITC)-conjugated phalloidin (P1951; Sigma) was used to label actin filaments according to the manufacturer’s instruction.

Li N., Babaei-Jadidi R., Lorenzi F., Spencer-Dene B., Clarke P., Domingo E., Tulchinsky E., Vries R.G., Kerr D., Pan Y., He Y., Bates D.O., Tomlinson I., Clevers H, & Nateri A.S. (2019). An FBXW7-ZEB2 axis links EMT and tumour microenvironment to promote colorectal cancer stem cells and chemoresistance. Oncogenesis, 8(3), 13.

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Antibody Sourcing for Cell Signaling

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Antibodies were purchased as follow: β-catenin (610154, BD-Transduction and 9582, Cell-Signalling), ZEB2/SIP1 (H260, Santa-Cruz), Vimentin (RV202, Santa-Cruz), ZEB1 (H-102 and E-20X, Santa-Cruz), E-cadherin (BD-Biosciences), TCF4 (Upstate; 05-511), FLYWCH1 (Sigma; HPA040753, Santa-Cruz; V18), GFP (3E6, Invitrogen), Flag-tag (F1804, Sigma), Myc-tag (9E10, Millipore) and β-Actin (Abcam).

Muhammad B.A., Almozyan S., Babaei-Jadidi R., Onyido E.K., Saadeddin A., Kashfi S.H., Spencer-Dene B., Ilyas M., Lourdusamy A., Behrens A, & Nateri A.S. (2018). FLYWCH1, a Novel Suppressor of Nuclear β-catenin, Regulates Migration and Morphology in Colorectal Cancer. Molecular cancer research : MCR, 16(12), 1977-1990.

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