Bcl 2 primary antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Bcl-2 primary antibody is a laboratory reagent used to detect the presence of the Bcl-2 protein in biological samples. Bcl-2 is an anti-apoptotic protein that plays a crucial role in regulating programmed cell death. This antibody can be used in various immunochemical techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to analyze Bcl-2 expression levels.

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Lab products found in correlation

Chemiluminescence kit, by Cytiva (1 mentions) Hrp conjugated goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) β actin antibody, by Boster Bio (1 mentions) Trypsinase, by Merck Group (1 mentions) High glucose dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Hoechst 33258 staining kit, by Beyotime (1 mentions) Eclipse e600, by Nikon (1 mentions)

Spelling variants of this product

Bcl-2 (950 citations) Bcl-2 antibody (22 citations) Primary antibody against Bcl-2 (2 citations)

4 protocols using bcl 2 primary antibody

Bcl-2 Immunofluorescence Staining Protocol

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After treatment with compound 28, the cells were washed
once with PBS and fixed with 3.7% paraformaldehyde at 37 °C for
15 min. The cells were further washed twice with PBS (pH = 7.4) and
permeabilized by incubating in blocking buffer (PBS containing 0.3%
Tween and 5% FBS) at room temperature. The cells were then incubated
in Bcl-2 primary antibody (Santa Cruz Biotechnology, Inc.) in 1:100
dilution at 37 °C for 3 h. The cells were washed thrice with
blocking buffer followed by incubation in Alexa Fluor 488 antimouse
IgG secondary antibody solution (1:500 dilutions) at 37 °C for
40 min in the dark. The cells were washed thrice with PBS and mounted
on a glass slide using SlowFade Gold antifade reagent. The slides
were subjected to fluorescence imaging using CLSM.

Patil S., Kuman M.M., Palvai S., Sengupta P, & Basu S. (2018). Impairing Powerhouse in Colon Cancer Cells by Hydrazide–Hydrazone-Based Small Molecule. ACS Omega, 3(2), 1470-1481.

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Protein Expression Analysis of Heat-Treated Cells

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Cells were heat treated to 1 time at temperature of 44˚C for 1h. After 24h incubation, cells were homogenized with RIPA lysis buffer. 30 μg protein was loaded in 12% SDS-polyacrylamide gel electrophoresis (PAGE). Electroblotting was performed using wet blotting system and blocking stage of mem-brane in 5% non-fat milk in TBST for an hour. Membrane containing protein was incubated with rabbit polyclonal β-actin primary antibody (1:200; Santa Cruz Biotechnology Inc., USA), rabbit polyclonal Bax primary antibody (1:200; Santa Cruz Biotechnology Inc., USA) and rabbit polyclonal Bcl-2 primary antibody (1:200; Santa Cruz Biotech-nology Inc., USA) overnight at 4°C. After washing with TBS-T, the membrane incubated with HRP-conjugated goat anti-rabbit IgG (1:10,000; Santa Cruz Biotechnology Inc., USA) for 1h in room temperature in the dark. Finally, protein was exposed using chemi-luminescence kit (Amersham Biosciences, Orsay, France) on an X-ray lm after a 10s exposure.

Hashemi M., Abbasiazam A., Oraee-Yazdani S, & Lenzer J. (2022). Response of human glioblastoma cells to hyperthermia: Cellular apoptosis and molecular events. Tissue & cell, 75.

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Isoproterenol-Induced Endoplasmic Reticulum Stress

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Isoproterenol (ISO), resveratrol (Res) and trypsinase were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.). High glucose Dulbecco's modified Eagle's medium (DMEM) was a product of Gibco BRL (Gaithersburg, MD, U.S.). Glucose-regulated protein 78 (GRP78), glucose-regulated protein 94 (GRP94), C/EBP homologousprotein (CHOP), Bax and Bcl-2 primary antibodies were obtained from Santa Cruz Biotechnology Incorporated (Santa Cruz, CA, U.S.). Trizol reagent and the PrimerScript RT reagent kit were purchased from TakaRa Biotechnology Incorporated (TakaRa Bio Inc., Japan). The western blot kit and β-actin antibody were purchased from Boster (Wuhan, China). Hoechst 33258 staining kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China). Assay kits for malondialdehyde (MDA) and lactate dehydrogenase (LDH) were purchased from Jiancheng Bioengineering Institute (Nanjing, China).

Lin Y., Zhu J., Zhang X., Wang J., Xiao W., Li B., Jin L., Lian J., Zhou L, & Liu J. (2016). Inhibition of Cardiomyocytes Hypertrophy by Resveratrol Is Associated with Amelioration of Endoplasmic Reticulum Stress. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 39(2).

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Histological and Immunohistochemical Analysis of Liver

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Liver samples were dissected out, excised from the experimental animals of each group, held with the normal saline, fixed in 10% formalin, and processed for paraffin embedding following the microtome technique. The sections were taken at 5 μm thickness, processed in alcohol-xylene series, and were stained with alum-haematoxylin and eosin. The sections were examined microscopically under 40x magnification for the evaluation of histopathological changes.
Sections (5 μm) were prepared and mounted on poly-l-lysine-coated slides. After deparaffinization, antigen retrieval was applied using citrate buffer (0.1 M, pH: 6.0). Hydrogen peroxide (3%) was used for peroxidase activity inhibition. Bcl-2 primary antibodies (Santa Cruz) were used for antigen detection in fixed tissues and probed with a secondary antibody provided by the HRP kit (Labvision). 3-Amino-9-ethylcarbazole (AEC) (Labvision) was used as a chromogen to counterstain the slides, which were evaluated under light microscopy (Nikon Eclipse E-600). For immunopositive cell counting, NIS 4.0 Nikon Image Analysis Software was utilized. The immunopositive cells in four different objective areas were counted under 100x magnification.

El-Baz F.K., Saleh D.O., Abdel Jaleel G.A, & Hussein R.A. (2020). Attenuation of Age-Related Hepatic Steatosis by Dunaliella salina Microalgae in Senescence Rats through the Regulation of Redox Status, Inflammatory Indices, and Apoptotic Biomarkers. Advances in Pharmacological and Pharmaceutical Sciences, 2020, 3797218.

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