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Phospho s473 kap1

Manufactured by BioLegend

Phospho-S473-KAP1 is a lab equipment product that detects the phosphorylation of the KAP1 (KRAB-associated protein 1) protein at serine 473. This product can be used to measure the phosphorylation status of KAP1, a protein involved in chromatin remodeling and transcriptional regulation.

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2 protocols using phospho s473 kap1

1

Comprehensive Western Blot Analysis

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Western blot analysis was conducted as described previously(23 (link)). Antibodies used were phospho-S473-KAP1 (644602, BioLegend), phospho-S824-KAP1 (A300-767A, Bethyl), KAP1 (A300-274A, Bethyl), β-actin (MAB1501R), H3K27me3 (07-449) from Millipore, Tom20 (sc-17764) and GAPDH (sc-25778) from Santa Cruz, phospho-T172-AMPKα (2535), AMPKα (2532), phospho-T180/Y182-p38 (9216) and p38 (9212) from Cell Signaling, MFN1 (GTX116254), MFN2 (GTX102055), OPA1 (GTX48589) and FIS1/TTC11 (GTX111010) from GeneTex, H3 (39763), H3K9me3 (61013) from Active Motif and Mitoprofile OXPHOS Cocktail (MS601) from MitoSciences.
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2

Antibody Panel for Cell Signaling

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The following antibodies were used: Phospho-S473-KAP1 (BioLegend Cat# 654102, RRID: AB_2561782), phospho-S516-CHK2 (Cell Signaling Technology Cat# 2669, RRID: AB_330146), CHK2 (Abcam Cat# ab109413, RRID: AB_10863751), KAP1 (GeneTex Cat# GTX102227, RRID: AB_2037323), PCNA (Santa Cruz Biotechnology Cat# sc-56, RRID:AB_628110), goat anti-mouse (Thermo Fisher Scientific Cat# A-11004, RRID: AB_2534072), and goat anti-rabbit Alexa568 (Thermo Fisher Scientific Cat# A-11011, RRID: AB_143157).
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