The total lysates were prepared in RIPA buffer plus protease inhibitors (Roche, 11836153001). Protein concentrations were determined by Bradford assay. Samples were separated by electrophoresis and transferred to a nitrocellulose membrane (Bio‐Rad, 1620115). The membrane was immunoblotted with anti‐mouse FTL/ferritin light chain antibody (Santa Cruz Biotechnology, sc‐74513), anti‐rabbit FTH/ferritin heavy chain antibody (Cell Signaling Technology, 4393S), anti‐rabbit total ferritin antibody (Sigma‐Aldrich, F5012), anti‐rabbit LC3B antibody (Cell Signaling Technology, 2775), anti‐rabbit SQSTM1/p62 (Cell Signaling Technology, 5114), anti‐rabbit WDR45 antibody (ProteinTech, 19194‐1‐AP), anti‐rabbit flag antibody (Sigma‐Aldrich, F7425), anti‐rabbit NCOA4 antibody (Bethyl Laboratories, A302272A), anti‐mouse CYCS/cytochrome c antibody (Santa Cruz Biotechnology, sc‐13156), and anti‐mouse ACTB/actin (Proteintech, 60008‐1‐Ig). The blots were visualized with infrared anti‐mouse or anti‐rabbit secondary antibodies, using a LI‐COR Odyssey fluorescent western blotting system (LI‐COR Biosciences). Protein expression was quantified using densitometry (Image Studio Lite; LI‐COR).
Anti mouse cycs cytochrome c antibody
Manufactured by Santa Cruz Biotechnology
The Anti‐mouse CYCS/cytochrome c antibody is a research-use only product designed for the detection of cytochrome c in mouse samples. This antibody is specific to the CYCS protein, which is a key component of the mitochondrial electron transport chain and plays a crucial role in cellular respiration and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti lc3b antibody, by Cell Signaling Technology (2 mentions)
Nitrocellulose membrane, by Bio-Rad (2 mentions)
Image studio lite, by LI COR (2 mentions)
Anti mouse ftl ferritin light chain antibody, by Santa Cruz Biotechnology (2 mentions)
Anti rabbit fth ferritin heavy chain antibody, by Cell Signaling Technology (2 mentions)
Anti rabbit total ferritin antibody, by Merck Group (2 mentions)
Anti rabbit wdr45 antibody, by Proteintech (2 mentions)
Anti rabbit ncoa4 antibody, by Fortis Life Sciences (2 mentions)
Anti mouse actb actin, by Proteintech (2 mentions)
Rabbit anti flag antibody, by Merck Group (2 mentions)
Rabbit anti sqstm1 p62, by Cell Signaling Technology (2 mentions)
Odyssey fluorescent western blotting system, by LI COR (2 mentions)
2 protocols using anti mouse cycs cytochrome c antibody
1
Ferritin and Autophagy Regulation Assay
2
Immunoblotting analysis of ferritin and autophagy
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