Mtt cell counting kit

Manufactured by Nacalai Tesque

Sourced in Japan

The MTT cell-counting kit is a laboratory reagent used to measure cell viability and proliferation. It works by colorimetric detection of the reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to formazan by metabolically active cells. The resulting color change can be quantified using a spectrophotometer, providing an indication of the number of viable cells in a sample.

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Lab products found in correlation

Spectramax i3, by Molecular Devices (1 mentions) Annexin 5 fitc apoptosis detection kit, by Nacalai Tesque (1 mentions) Fluosphere, by Thermo Fisher Scientific (1 mentions) Fluorescent beads, by Thermo Fisher Scientific (1 mentions)

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Cell counting kit (3 citations)

3 protocols using mtt cell counting kit

MTT Assay for Aβo-Induced Cytotoxicity

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Assessment of the effects of curcumin and GT863 on the cell viability of SH-SY5Y cells exposed to Aβo was measured using the MTT cell-counting kit (Nacalai Tesque, Inc., Kyoto, Japan). First, a preliminary experiment was performed in which differentiated SH-SY5Y cells (1 × 10⁶ cells/mL) were cultured in collagen-coated 96-well plates and exposed to Aβo (1, 5, 10 µM) for 3 h. As shown in Figure 3A, the appropriate Aβo concentration to induce cytotoxicity was 5 µM.
Next, to investigate the protective effects of curcumin and GT863 on Aβo-induced cytotoxicity, SH-SY5Y cells were treated with Aβo + curcumin (1, 10 µM) or Aβo + GT863 (1, 10 µM) for 3 h. After incubation, the MTT assay was performed and measured at 570 nm using a Spectra Max i3 (Molecular Devices) microplate reader.

Momma Y., Tsuji M., Oguchi T., Ohashi H., Nohara T., Ito N., Yamamoto K., Nagata M., Kimura A.M., Nakamura S., Kiuchi Y, & Ono K. (2023). The Curcumin Derivative GT863 Protects Cell Membranes in Cytotoxicity by Aβ Oligomers. International Journal of Molecular Sciences, 24(4), 3089.

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Apoptosis Imaging Using Glutathione-Coated Quantum Dots

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Glutathione-coated CdSeTe/CdS
QDs (GSH–QDs) and annexinV–EGFP were prepared according
to the previously reported methods (Supporting Information).^{64 (link),65 (link)} The anti-HER2 monoclonal antibody
(Herceptin) and Kadcyla (trastuzumab emtansine) were purchased from
Chugai Pharmaceutical Co. Ltd. (Japan). The FITC-annexin V apoptosis
detection kit and MTT cell counting kit were purchased from Nacalai
Tesque (Japan). Fluorescent beads (size: 14 nm in diameter, latex,
FluoSpheres, carboxylate-modified and red fluorescent) were purchased
from Molecular Probes, Inc. Soybean lecithin was purchased from Nacalai
Tesque. l-α-Phosphatidyl-l-serine (soybean)
was purchased from Sigma. All other regents were of analytical grade
and were used as received without further purification. Breast tumor
cells (KPL-4) were kindly provided by Dr. J. Kurebayashi (Kawasaki
Medical School). Nude mice (5 week old female BALB/c nu/nu) were purchased
from Nihon SLC Inc. (Japan).

Tsuboi S, & Jin T. (2022). In Vitro and In Vivo Fluorescence Imaging of Antibody–Drug Conjugate-Induced Tumor Apoptosis Using Annexin V–EGFP Conjugated Quantum Dots. ACS Omega, 7(2), 2105-2113.

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Evaluating Cytotoxicity of Trovafloxacin and TNF-α in HepG2 Cells

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HepG2 cells were seeded into 96-well plates at 1 × 10⁴ cells/well and were maintained at 37°C for 24 hours. Twenty-four hours after changing the culture medium to a normal or a chrysin-containing DMEM medium, the cells were co-treated with trovafloxacin (50 μM) and TNF-α (4ng/mL) for 24 hours. Cell viability was measured using a MTT Cell Counting Kit (Nacalai Tesque) according to the manufacture’s protocol.

Mitsugi R., Sumida K., Fujie Y., Tukey R.H., Itoh T, & Fujiwara R. (2016). Trovafloxacin acyl-glucuronide induces chemokine (C-X-C motif) ligand 2 in HepG2 cells. Biological & pharmaceutical bulletin, 39(10), 1604-1610.

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