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2 protocols using anti pkd pkcμ

1

Signaling Pathway Reagents and Antibodies

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SDF-1α and CSF-1 were purchased from R&D Systems (Minneapolis, MN) and Pepro Tech (Rocky Hill, NJ), respectively. Gö 6976, Gö 6983, and U73122 were purchased from Calbiochem (Billerica, MA). CID755673 was purchased from Tocris (United Kingdom). fMLP and DMSO were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies anti–phospho-PKD/PKCmu(S744/748), anti–phospho-PKD1(Ser-916), and anti-PKD/PKCμ were from Cell Signaling (Danvers, MA). Anti-SSH2 antibody was from Novus Biologicals. Anti–14‑3‑3 antibody was from Santa Cruz Biotechnology. Anti-GFP antibody was from BD Bioscience (San Jose, CA). Horseradish peroxidase (HRP)–conjugated anti-actin was from Santa Cruz Biotechnology (Dallas, TX). HRP-conjugated anti-mouse or anti-rabbit immunoglobulin G was obtained from Jackson Immuno­Research (United Kingdom). All tissue culture reagents were purchased from Invitrogen.
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2

Antibody Detection of ERK and PKD

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The antibodies used for this study were anti-ERK (catalog Sc-94), anti-phospho-ERK (Y204) (catalog Sc-7383), from Santa Cruz Biotechnology, Inc., (Dallas, TX, USA). Anti-PKD/PKCμ (catalog 2052), and anti- phospho- PKD/PKCμ (S916) (catalog 2054) were obtained from Cell Signaling Technology (Danvers, MA, USA).
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